› 8tj3

Electron Microscopy
3.2Å resolution

Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex

Released:
DOI: 10.2210/pdb8tj3/pdb
PDB entry 8tj3 coloured by chain, front view.
PDB entry 8tj3 coloured by chain, side view.
PDB entry 8tj3 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex.
Nygaard R, Graham CLB, Belcher Dufrisne M, Colburn JD, Pepe J, Hydorn MA, Corradi S, Brown CM, Ashraf KU, Vickery ON, Briggs NS, Deering JJ, Kloss B, Botta B, Clarke OB, Columbus L, Dworkin J, Stansfeld PJ, Roper DI, Mancia F
OpenAccess logo Nat Commun 14 5151 (2023)
PMID: 37620344
Related structures: EMD-41299

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-272630 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peptidoglycan glycosyltransferase MrdB Chain: B
Molecule details ›
Chain: B
Length: 370 amino acids
Theoretical weight: 40.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABG7 (Residues: 1-370; Coverage: 100%)
Gene names: JW0629, b0634, mrdB, rodA
Sequence domains: Cell cycle protein
Peptidoglycan D,D-transpeptidase MrdA Chain: C
Molecule details ›
Chain: C
Length: 633 amino acids
Theoretical weight: 70.94 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AD65 (Residues: 1-633; Coverage: 100%)
Gene names: JW0630, b0635, mrdA, pbpA
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-41299
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Diversity of sugar-diphospholipid-utilizing glycosyltransferase families.
Meitil et al. (2024)