› 8tj4

X-ray diffraction
1.95Å resolution

CRYSTAL STRUCTURE OF THE A/Bangkok/1/1979(H3N2) INFLUENZA VIRUS HEMAGGLUTININ WITH HUMAN RECEPTOR ANALOG 6'-SLNLN

Released:
DOI: 10.2210/pdb8tj4/pdb
PDB entry 8tj4 coloured by chain, front view.
PDB entry 8tj4 coloured by chain, side view.
PDB entry 8tj4 coloured by chain, top view.
Source organism: Influenza A virus (A/Bangkok/1/1979(H3N2))
Primary publication:
Evolution of human H3N2 influenza virus receptor specificity has substantially expanded the receptor-binding domain site.
Thompson AJ, Wu NC, Canales A, Kikuchi C, Zhu X, de Toro BF, Cañada FJ, Worth C, Wang S, McBride R, Peng W, Nycholat CM, Jiménez-Barbero J, Wilson IA, Paulson JC
Cell Host Microbe (2024)
PMID: 38307019

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-272214 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (9 distinct):
Hemagglutinin HA1 chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 323 amino acids
Theoretical weight: 35.72 KDa
Source organism: Influenza A virus (A/Bangkok/1/1979(H3N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P03441 (Residues: 11-329; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Hemagglutinin HA2 chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 174 amino acids
Theoretical weight: 20.06 KDa
Source organism: Influenza A virus (A/Bangkok/1/1979(H3N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P03441 (Residues: 330-503; Coverage: 32%)
Gene name: HA
Sequence domains: Haemagglutinin

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, GAL, SIA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GAL, SIA
Carbohydrate polymer
4 bound ligands:
Ligand NAG 7 x NAG
Ligand PG4 2 x PG4
Ligand PG0 2 x PG0
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: R3
Unit cell:
a: 100.655Å b: 100.655Å c: 687.903Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.189 0.223
Expression system: Trichoplusia ni

Quick links

Citations

1 citations in other articles

Epistasis mediates the evolution of the receptor binding mode in recent human H3N2 hemagglutinin.
Lei et al. (2024)