› 8tjh

X-ray diffraction
2Å resolution

TGP-E, extreme thermostable green fluorescent protein (TGP) with Q66E mutation

Released:
DOI: 10.2210/pdb8tjh/pdb
PDB entry 8tjh coloured by chain, front view.
PDB entry 8tjh coloured by chain, side view.
PDB entry 8tjh coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
The Crystal Structure of Thermal Green Protein Q66E (TGP-E) and Yellow Thermostable Protein (YTP-E) E148D
Anderson MR, Padgett CM, Ogbeifun VO, DeVore NM
SynBio 2 298-310 (2024)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-247427 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
extreme thermostable green fluorescent protein (TGP-E) Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 28.25 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRU 2 x CRU

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P1
Unit cell:
a: 38.736Å b: 49.064Å c: 59.108Å
α: 78.55° β: 72.08° γ: 71.41°
R-values:
R R work R free
0.172 0.169 0.229
Expression system: Escherichia coli BL21(DE3)

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