› 8tk2

X-ray diffraction
1.7Å resolution

HUMAN VH1-RELATED DUAL-SPECIFICITY PHOSPHATASE (VHR) complexed with 2-((2,4-difluorobenzyl)amino)-2-oxoacetic acid

Released:
DOI: 10.2210/pdb8tk2/pdb
PDB entry 8tk2 coloured by chain, front view.
PDB entry 8tk2 coloured by chain, side view.
PDB entry 8tk2 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Aleshin AE, Wu J, Lambert LJ, Cosford NDP, Tautz L

Function and Biology Details

Reactions catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156340 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity protein phosphatase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 20.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P51452 (Residues: 3-185; Coverage: 99%)
Gene names: DUSP3, VHR
Sequence domains: Dual specificity phosphatase, catalytic domain

Ligands and Environments

3 bound ligands:
Ligand I1Y 2 x I1Y
Ligand DMS 1 x DMS
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P1
Unit cell:
a: 35.536Å b: 44.328Å c: 59.863Å
α: 78.07° β: 89.99° γ: 81.69°
R-values:
R R work R free
0.179 0.176 0.223
Expression system: Escherichia coli BL21(DE3)

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