› 8tk7

Electron Microscopy
2.53Å resolution

Myxococcus xanthus EncA protein shell with compartmentalized SNAP-tag cargo protein

Released:
DOI: 10.2210/pdb8tk7/pdb
PDB entry 8tk7 coloured by chain, front view.
PDB entry 8tk7 coloured by chain, side view.
PDB entry 8tk7 coloured by chain, top view.
Source organisms:
Primary publication:
Structure and heterogeneity of a highly cargo-loaded encapsulin shell.
Kwon S, Andreas MP, Giessen TW
J Struct Biol 108022 (2023)
PMID: 37657675
Related structures: EMD-41322

Function and Biology Details

Reaction catalysed: 
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 360-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-513893 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Type 1 encapsulin shell protein EncA Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 287 amino acids
Theoretical weight: 31.69 KDa
Source organism: Myxococcus xanthus DK 1622
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1D6H4 (Residues: 1-287; Coverage: 100%)
Gene names: MXAN_3556, encA
Sequence domains: Encapsulating protein for peroxidase
Methylated-DNA--protein-cysteine methyltransferase Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 203 amino acids
Theoretical weight: 21.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E5BBQ0 (Residues: 1-182; Coverage: 100%)
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.53Å
Relevant EMDB volumes: EMD-41322
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Structure and heterogeneity of a highly cargo-loaded encapsulin shell.
Kwon et al. (2023)