› 8tkb

X-ray diffraction
1.71Å resolution

tRNA 2-phosphotransferase (Tpt1) from Pyrococcus horikoshii in complex with 5'-AMP

Released:
DOI: 10.2210/pdb8tkb/pdb
PDB entry 8tkb coloured by chain, front view.
PDB entry 8tkb coloured by chain, side view.
PDB entry 8tkb coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Jacewicz A, Dantuluri S, Shuman S

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-234993 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable RNA 2'-phosphotransferase Chain: A
Molecule details ›
Chain: A
Length: 177 amino acids
Theoretical weight: 20.84 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O57899 (Residues: 2-177; Coverage: 99%)
Gene names: PH0160, kptA
Sequence domains: RNA 2'-phosphotransferase, Tpt1 / KptA family

Ligands and Environments

4 bound ligands:
Ligand AMP 2 x AMP
Ligand K 4 x K
Ligand CL 7 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 38.937Å b: 43.665Å c: 122.861Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.209
Expression system: Escherichia coli BL21(DE3)

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