8tl4

Electron Microscopy
3.2Å resolution

CRYO-EM STRUCTURE OF HIV-1 BG505DS-SOSIP.664 ENV TRIMER BOUND TO DJ85-e.01 FAB

Released:
Entry authors: Pletnev S, Hoyt F, Fischer E, Kwong P
Related structures: EMD-41361

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-250818 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
Envelope glycoprotein gp160 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 481 amino acids
Theoretical weight: 54.09 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S5 (Residues: 30-508; Coverage: 56%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Transmembrane protein gp41 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 153 amino acids
Theoretical weight: 17.15 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S5 (Residues: 509-661; Coverage: 18%)
Gene name: env
Sequence domains: Retroviral envelope protein
DJ85-e.01 FAB HEAVY CHAIN Chains: G, I, K
Molecule details ›
Chains: G, I, K
Length: 236 amino acids
Theoretical weight: 25.35 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
DJ85-e.01 FAB LIGHT CHAIN Chains: H, J, L
Molecule details ›
Chains: H, J, L
Length: 214 amino acids
Theoretical weight: 23.53 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-41361
Expression system: Homo sapiens