› 8tlh

X-ray diffraction
1.94Å resolution

CDCA7 (Mouse) Binds Non-B-form 32-mer DNA oligo

Released:
DOI: 10.2210/pdb8tlh/pdb
PDB entry 8tlh coloured by chain, front view.
PDB entry 8tlh coloured by chain, side view.
PDB entry 8tlh coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The ICF syndrome protein CDCA7 harbors a unique DNA-binding domain that recognizes a CpG dyad in the context of a non-B DNA.
Hardikar S, Ren R, Ying Z, Horton JR, Bramble MD, Liu B, Lu Y, Liu B, Dan J, Zhang X, Cheng X, Chen T
bioRxiv (2023)
PMID: 38168392

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270796 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Cell division cycle-associated protein 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 144 amino acids
Theoretical weight: 16.45 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9D0M2 (Residues: 242-382; Coverage: 37%)
Gene name: Cdca7
Sequence domains: Zinc-finger domain of monoamine-oxidase A repressor R1
DNA (32-MER) Chains: C, X
Molecule details ›
Chains: C, X
Length: 32 nucleotides
Theoretical weight: 9.85 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand EDO 6 x EDO
Ligand ZN 6 x ZN
Ligand GOL 2 x GOL
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 169.679Å b: 31.523Å c: 91.634Å
α: 90° β: 110.59° γ: 90°
R-values:
R R work R free
0.2 0.199 0.227
Expression systems:
  • Escherichia coli
  • Not provided

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