› 8tlr

X-ray diffraction
1.7Å resolution

Crystal Structure of human HRAS G12C covalently bound to AMG 510

Released:
DOI: 10.2210/pdb8tlr/pdb
Model geometry
Fit model/data
PDB entry 8tlr coloured by chain, front view.
PDB entry 8tlr coloured by chain, side view.
PDB entry 8tlr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Targeting Ras-, Rho-, and Rab-family GTPases via a conserved cryptic pocket.
Morstein J, Bowcut V, Fernando M, Yang Y, Zhu L, Jenkins ML, Evans JT, Guiley KZ, Peacock DM, Krahnke S, Lin Z, Taran KA, Huang BJ, Stephen AG, Burke JE, Lightstone FC, Shokat KM
Cell (2024)
PMID: 39255801

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family

Ligands and Environments

3 bound ligands:
Ligand GDP 1 x GDP
Ligand MOV 1 x MOV
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: R32
Unit cell:
a: 92.449Å b: 92.449Å c: 119.711Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.184 0.21
Expression system: Escherichia coli

Quick links

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO