› 8tm3

Electron Microscopy
3Å resolution

Human proteasome alpha ring assembly intermediate

Released:
DOI: 10.2210/pdb8tm3/pdb
PDB entry 8tm3 coloured by chain, front view.
PDB entry 8tm3 coloured by chain, side view.
PDB entry 8tm3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of human 20S proteasome biogenesis.
Zhang H, Zhou C, Mohammad Z, Zhao J
Nat Commun 15 8184 (2024)
PMID: 39294158
Related structures: EMD-41377

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-279324 (preferred)
Entry contents:
11 distinct polypeptide molecules
Macromolecules (11 distinct):
Proteasome subunit alpha type-2 Chain: A
Molecule details ›
Chain: A
Length: 234 amino acids
Theoretical weight: 25.93 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25787 (Residues: 1-234; Coverage: 100%)
Gene names: HC3, PSC3, PSMA2
Sequence domains:
Proteasome subunit alpha type-4 Chain: B
Molecule details ›
Chain: B
Length: 261 amino acids
Theoretical weight: 29.53 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25789 (Residues: 1-261; Coverage: 100%)
Gene names: HC9, PSC9, PSMA4
Sequence domains:
Proteasome subunit alpha type-7 Chain: C
Proteasome subunit alpha type-5 Chain: D
Proteasome subunit alpha type-1 Chain: E
Proteasome subunit alpha type-3 Chain: F
Proteasome subunit alpha type-6 Chain: G
Proteasome assembly chaperone 1 Chain: c
Proteasome assembly chaperone 2 Chain: d
Proteasome assembly chaperone 3 Chain: i
Proteasome assembly chaperone 4 Chain: j

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3Å
Relevant EMDB volumes: EMD-41377

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