› 8tm3

Electron Microscopy
3Å resolution

Human proteasome alpha ring assembly intermediate

Released:
DOI: 10.2210/pdb8tm3/pdb
PDB entry 8tm3 coloured by chain, front view.
PDB entry 8tm3 coloured by chain, side view.
PDB entry 8tm3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of human 20S proteasome biogenesis.
Zhang H, Zhou C, Mohammad Z, Zhao J
Nat Commun 15 8184 (2024)
PMID: 39294158
Related structures: EMD-41377

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-279324 (preferred)
Entry contents:
11 distinct polypeptide molecules
Macromolecules (11 distinct):
Proteasome subunit alpha type-2 Chain: A
Molecule details ›
Chain: A
Length: 234 amino acids
Theoretical weight: 25.93 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25787 (Residues: 1-234; Coverage: 100%)
Gene names: HC3, PSC3, PSMA2
Sequence domains:
Proteasome subunit alpha type-4 Chain: B
Molecule details ›
Chain: B
Length: 261 amino acids
Theoretical weight: 29.53 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25789 (Residues: 1-261; Coverage: 100%)
Gene names: HC9, PSC9, PSMA4
Sequence domains:
Proteasome subunit alpha type-7 Chain: C
Molecule details ›
Chain: C
Length: 248 amino acids
Theoretical weight: 27.93 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O14818 (Residues: 1-248; Coverage: 100%)
Gene names: HSPC, PSMA7
Sequence domains:
Proteasome subunit alpha type-5 Chain: D
Molecule details ›
Chain: D
Length: 241 amino acids
Theoretical weight: 26.44 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28066 (Residues: 1-241; Coverage: 100%)
Gene name: PSMA5
Sequence domains:
Proteasome subunit alpha type-1 Chain: E
Molecule details ›
Chain: E
Length: 263 amino acids
Theoretical weight: 29.6 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25786 (Residues: 1-263; Coverage: 100%)
Gene names: HC2, NU, PROS30, PSC2, PSMA1
Sequence domains:
Proteasome subunit alpha type-3 Chain: F
Molecule details ›
Chain: F
Length: 255 amino acids
Theoretical weight: 28.47 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25788 (Residues: 1-255; Coverage: 100%)
Gene names: HC8, PSC8, PSMA3
Sequence domains:
Proteasome subunit alpha type-6 Chain: G
Molecule details ›
Chain: G
Length: 246 amino acids
Theoretical weight: 27.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60900 (Residues: 1-246; Coverage: 100%)
Gene names: PROS27, PSMA6
Sequence domains:
Proteasome assembly chaperone 1 Chain: c
Molecule details ›
Chain: c
Length: 288 amino acids
Theoretical weight: 32.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O95456 (Residues: 1-288; Coverage: 100%)
Gene names: C21LRP, DSCR2, PAC1, PSMG1
Sequence domains: Proteasome assembly chaperone 4
Proteasome assembly chaperone 2 Chain: d
Molecule details ›
Chain: d
Length: 264 amino acids
Theoretical weight: 29.42 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q969U7 (Residues: 1-264; Coverage: 100%)
Gene names: HCCA3, PAC2, PSMG2, TNFSF5IP1
Sequence domains: PAC2 family
Proteasome assembly chaperone 3 Chain: i
Molecule details ›
Chain: i
Length: 122 amino acids
Theoretical weight: 13.12 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BT73 (Residues: 1-122; Coverage: 100%)
Gene names: C7orf48, PAC3, PSMG3
Sequence domains: Proteasome assembly chaperone 3
Proteasome assembly chaperone 4 Chain: j
Molecule details ›
Chain: j
Length: 123 amino acids
Theoretical weight: 13.79 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q5JS54 (Residues: 1-123; Coverage: 100%)
Gene names: C6orf86, PAC4, PSMG4
Sequence domains: Proteasome assembly chaperone 4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-41377

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