› 8tm6

Electron Microscopy
2.8Å resolution

Human premature 20S proteasome assembly intermediate

Released:
DOI: 10.2210/pdb8tm6/pdb
PDB entry 8tm6 coloured by chain, front view.
PDB entry 8tm6 coloured by chain, side view.
PDB entry 8tm6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of human 20S proteasome biogenesis.
Zhang H, Zhou C, Mohammad Z, Zhao J
Nat Commun 15 8184 (2024)
PMID: 39294158
Related structures: EMD-41380

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 34-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-271561 (preferred)
Entry contents:
17 distinct polypeptide molecules
Macromolecules (17 distinct):
Proteasome subunit alpha type-2 Chains: A, O
Molecule details ›
Chains: A, O
Length: 234 amino acids
Theoretical weight: 25.93 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25787 (Residues: 1-234; Coverage: 100%)
Gene names: HC3, PSC3, PSMA2
Sequence domains:
Proteasome subunit alpha type-4 Chains: B, P
Molecule details ›
Chains: B, P
Length: 261 amino acids
Theoretical weight: 29.53 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25789 (Residues: 1-261; Coverage: 100%)
Gene names: HC9, PSC9, PSMA4
Sequence domains:
Proteasome subunit alpha type-7 Chains: C, Q
Molecule details ›
Chains: C, Q
Length: 248 amino acids
Theoretical weight: 27.93 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O14818 (Residues: 1-248; Coverage: 100%)
Gene names: HSPC, PSMA7
Sequence domains:
Proteasome subunit alpha type-5 Chains: D, R
Molecule details ›
Chains: D, R
Length: 241 amino acids
Theoretical weight: 26.44 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28066 (Residues: 1-241; Coverage: 100%)
Gene name: PSMA5
Sequence domains:
Proteasome subunit alpha type-1 Chains: E, S
Molecule details ›
Chains: E, S
Length: 263 amino acids
Theoretical weight: 29.6 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25786 (Residues: 1-263; Coverage: 100%)
Gene names: HC2, NU, PROS30, PSC2, PSMA1
Sequence domains:
Proteasome subunit alpha type-3 Chains: F, T
Molecule details ›
Chains: F, T
Length: 255 amino acids
Theoretical weight: 28.47 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25788 (Residues: 1-255; Coverage: 100%)
Gene names: HC8, PSC8, PSMA3
Sequence domains:
Proteasome subunit alpha type-6 Chains: G, U
Molecule details ›
Chains: G, U
Length: 246 amino acids
Theoretical weight: 27.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60900 (Residues: 1-246; Coverage: 100%)
Gene names: PROS27, PSMA6
Sequence domains:
Proteasome subunit beta type-7 Chains: H, V
Molecule details ›
Chains: H, V
Length: 277 amino acids
Theoretical weight: 30 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q99436 (Residues: 1-277; Coverage: 100%)
Gene names: PSMB7, Z
Sequence domains:
Proteasome subunit beta type-3 Chains: I, W
Molecule details ›
Chains: I, W
Length: 205 amino acids
Theoretical weight: 22.97 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49720 (Residues: 1-205; Coverage: 100%)
Gene name: PSMB3
Sequence domains: Proteasome subunit
Proteasome subunit beta type-2 Chains: J, X
Molecule details ›
Chains: J, X
Length: 201 amino acids
Theoretical weight: 22.86 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49721 (Residues: 1-201; Coverage: 100%)
Gene name: PSMB2
Sequence domains: Proteasome subunit
Proteasome subunit beta type-5 Chains: K, Y
Molecule details ›
Chains: K, Y
Length: 263 amino acids
Theoretical weight: 28.51 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28074 (Residues: 1-263; Coverage: 100%)
Gene names: LMPX, MB1, PSMB5, X
Sequence domains: Proteasome subunit
Proteasome subunit beta type-1 Chains: L, Z
Molecule details ›
Chains: L, Z
Length: 241 amino acids
Theoretical weight: 26.52 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P20618 (Residues: 1-241; Coverage: 100%)
Gene names: PSC5, PSMB1
Sequence domains: Proteasome subunit
Proteasome subunit beta type-4 Chains: M, a
Molecule details ›
Chains: M, a
Length: 264 amino acids
Theoretical weight: 29.23 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28070 (Residues: 1-264; Coverage: 100%)
Gene names: PROS26, PSMB4
Sequence domains: Proteasome subunit
Proteasome subunit beta type-6 Chains: N, b
Molecule details ›
Chains: N, b
Length: 239 amino acids
Theoretical weight: 25.38 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28072 (Residues: 1-239; Coverage: 100%)
Gene names: LMPY, PSMB6, Y
Sequence domains: Proteasome subunit
Proteasome assembly chaperone 1 Chains: c, f
Molecule details ›
Chains: c, f
Length: 288 amino acids
Theoretical weight: 32.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O95456 (Residues: 1-288; Coverage: 100%)
Gene names: C21LRP, DSCR2, PAC1, PSMG1
Sequence domains: Proteasome assembly chaperone 4
Proteasome assembly chaperone 2 Chains: d, g
Molecule details ›
Chains: d, g
Length: 264 amino acids
Theoretical weight: 29.42 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q969U7 (Residues: 1-264; Coverage: 100%)
Gene names: HCCA3, PAC2, PSMG2, TNFSF5IP1
Sequence domains: PAC2 family
Proteasome maturation protein Chains: e, h
Molecule details ›
Chains: e, h
Length: 141 amino acids
Theoretical weight: 15.8 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y244 (Residues: 1-141; Coverage: 100%)
Gene names: C13orf12, HSPC014, HSPC036, PNAS-110, POMP, UMP1
Sequence domains: Proteasome maturation factor UMP1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-41380

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