› 8tmg

Electron Microscopy
3Å resolution

Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-2A

Released:
DOI: 10.2210/pdb8tmg/pdb
PDB entry 8tmg coloured by chain, front view.
PDB entry 8tmg coloured by chain, side view.
PDB entry 8tmg coloured by chain, top view.
Source organisms:
Entry authors: Erramilli SK, Perozo E, Kossiakoff AA
Related structures: EMD-41388

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-285704 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
sAB C18 Heavy Chain Chains: F, H
Molecule details ›
Chains: F, H
Length: 237 amino acids
Theoretical weight: 25.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
sAB C18 Light Chain Chains: G, L
Molecule details ›
Chains: G, L
Length: 215 amino acids
Theoretical weight: 23.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
Cobalt/magnesium transport protein CorA Chains: A, B, C, D, E

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-41388
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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