8tni

Electron Microscopy
3.61Å resolution

Cryo-EM structure of HIV-1 Env BG505 DS-SOSIP in complex with broadly neutralizing bi-specific antibody CAP256L-R27 targeting the CD4-binding site and the V2-apex

Released:
Entry authors: Zhou T, Morano NC, Roark RS, Kwong PD, Xu J
Related structures: EMD-41417

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-244257 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (7 distinct):
Envelope glycoprotein gp160 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 481 amino acids
Theoretical weight: 54.09 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S6 (Residues: 30-505; Coverage: 55%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Transmembrane protein gp41 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 153 amino acids
Theoretical weight: 17.15 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S6 (Residues: 509-661; Coverage: 18%)
Gene name: env
Sequence domains: Retroviral envelope protein
Light chain of bi-specific antibody CAP256L-R27 Chains: H, I, J
Molecule details ›
Chains: H, I, J
Length: 353 amino acids
Theoretical weight: 36.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Heavy chain of bi-specific antibody CAP256L-R27 Chain: X
Molecule details ›
Chain: X
Length: 247 amino acids
Theoretical weight: 26.86 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.61Å
Relevant EMDB volumes: EMD-41417
Expression system: Homo sapiens