› 8tpj

Electron Microscopy
2.1Å resolution

Top cylinder bound to OCP from high-resolution phycobilisome quenched by OCP (local refinement)

Released:
DOI: 10.2210/pdb8tpj/pdb
PDB entry 8tpj coloured by chain, front view.
PDB entry 8tpj coloured by chain, side view.
PDB entry 8tpj coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Structural and quantum chemical basis for OCP-mediated quenching of phycobilisomes.
Sauer PV, Cupellini L, Sutter M, Bondanza M, Domínguez Martin MA, Kirst H, Bína D, Koh AF, Kotecha A, Greber BJ, Nogales E, Polívka T, Mennucci B, Kerfeld CA
OpenAccess logo Sci Adv 10 eadk7535 (2024)
PMID: 38578996
Related structures: EMD-41475

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
PDBe Complex ID:
PDB-CPX-246402 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Phycobiliprotein ApcE Chain: A
Molecule details ›
Chain: A
Length: 896 amino acids
Theoretical weight: 100.41 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: Q55544 (Residues: 1-896; Coverage: 100%)
Gene names: apcE, slr0335
Sequence domains:
Orange carotenoid-binding protein Chains: B, b
Molecule details ›
Chains: B, b
Length: 317 amino acids
Theoretical weight: 34.68 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P74102 (Residues: 1-317; Coverage: 100%)
Gene name: slr1963
Sequence domains:
Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core Chain: C
Molecule details ›
Chain: C
Length: 67 amino acids
Theoretical weight: 7.82 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: Q01950 (Residues: 1-67; Coverage: 100%)
Gene names: apcC, ssr3383
Sequence domains: CpcD/allophycocyanin linker domain
Allophycocyanin alpha chain Chains: D, F, H, J, L, N
Molecule details ›
Chains: D, F, H, J, L, N
Length: 161 amino acids
Theoretical weight: 17.43 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: Q01951 (Residues: 1-161; Coverage: 100%)
Gene names: apcA, slr2067
Sequence domains: Phycobilisome protein
Allophycocyanin beta chain Chains: E, G, I, K, M, O
Molecule details ›
Chains: E, G, I, K, M, O
Length: 161 amino acids
Theoretical weight: 17.25 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: Q01952 (Residues: 1-161; Coverage: 100%)
Gene names: apcB, slr1986
Sequence domains: Phycobilisome protein
Phycobilisome rod-core linker polypeptide CpcG Chains: P, Q, R, S
Molecule details ›
Chains: P, Q, R, S
Length: 249 amino acids
Theoretical weight: 28.94 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: P73093 (Residues: 1-249; Coverage: 100%)
Gene names: cpcG, cpcG1, slr2051
Sequence domains: Phycobilisome Linker polypeptide

Ligands and Environments

2 bound ligands:
Ligand 45D 1 x 45D
Ligand CYC 12 x CYC
1 modified residue:
Ligand MEN 6 x MEN

Experiments and Validation Details

Entry percentile scores
Resolution: 2.1Å
Relevant EMDB volumes: EMD-41475
Expression system: Escherichia coli BL21(DE3)

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