8tq9

X-ray diffraction
2.9Å resolution

Crystal structure of Fab.S19.8 in complex with MHC-I (H2-Dd)

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-241879 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
H-2 class I histocompatibility antigen, D-D alpha chain Chain: A
Molecule details ›
Chain: A
Length: 273 amino acids
Theoretical weight: 31.82 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01900 (Residues: 26-298; Coverage: 80%)
Gene name: H2-D1
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 101 amino acids
Theoretical weight: 11.89 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01887 (Residues: 19-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Fab.S19.8 Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 218 amino acids
Theoretical weight: 23.47 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Fab.S19.8 Light Chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.62 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Surface protein gp120 Chain: P
Molecule details ›
Chain: P
Length: 10 amino acids
Theoretical weight: 1.08 KDa
Source organism: HIV-1 M:B_HXB2R
Expression system: Not provided
UniProt:
  • Canonical: P04578 (Residues: 311-320; Coverage: 1%)
Gene name: env

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I222
Unit cell:
a: 54.901Å b: 183.181Å c: 216.715Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Mus musculus
  • Not provided