› 8tqc

Electron Microscopy
3.8Å resolution

Structure of the human CDK8 kinase module

Released:
DOI: 10.2210/pdb8tqc/pdb
PDB entry 8tqc coloured by chain, front view.
PDB entry 8tqc coloured by chain, side view.
PDB entry 8tqc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of the human transcriptional Mediator regulated by its dissociable kinase module.
Chao TC, Chen SF, Kim HJ, Tang HC, Tseng HC, Xu A, Palao L, Khadka S, Li T, Huang MF, Lee DF, Murakami K, Boyer TG, Tsai KL
Mol Cell (2024)
PMID: 39321804
Related structures: EMD-41502

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-273423 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Mediator of RNA polymerase II transcription subunit 12 Chain: C
Molecule details ›
Chain: C
Length: 2177 amino acids
Theoretical weight: 243.35 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: Q93074 (Residues: 1-2177; Coverage: 100%)
  • Best match: Q93074-2 (Residues: 1-2180)
Gene names: ARC240, CAGH45, HOPA, KIAA0192, MED12, TNRC11, TRAP230
Sequence domains:
Mediator of RNA polymerase II transcription subunit 13 Chain: D
Molecule details ›
Chain: D
Length: 2174 amino acids
Theoretical weight: 239.54 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: Q9UHV7 (Residues: 1-2174; Coverage: 100%)
Gene names: ARC250, KIAA0593, MED13, THRAP1, TRAP240
Sequence domains:
Cyclin-dependent kinase 8 Chain: A
Molecule details ›
Chain: A
Length: 464 amino acids
Theoretical weight: 53.37 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: P49336 (Residues: 1-464; Coverage: 100%)
Gene name: CDK8
Sequence domains: Protein kinase domain
Cyclin-C Chain: B
Molecule details ›
Chain: B
Length: 283 amino acids
Theoretical weight: 33.28 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: P24863 (Residues: 1-283; Coverage: 100%)
Gene name: CCNC
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-41502
Expression system: Baculovirus expression vector pFastBac1-HM

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