› 8trg

Electron Microscopy
2.93Å resolution

Structure of full-length LexA bound to a RecA filament

Released:
DOI: 10.2210/pdb8trg/pdb
PDB entry 8trg coloured by chain, front view.
PDB entry 8trg coloured by chain, side view.
PDB entry 8trg coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The LexA-RecA* structure reveals a cryptic lock-and-key mechanism for SOS activation.
Cory MB, Li A, Hurley CM, Carman PJ, Pumroy RA, Hostetler ZM, Perez RM, Venkatesh Y, Li X, Gupta K, Petersson EJ, Kohli RM
Nat Struct Mol Biol (2024)
PMID: 38755298
Related structures: EMD-41579

Function and Biology Details

Reaction catalysed: 
Hydrolysis of Ala(84)-|-Gly bond in repressor LexA.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235667 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
Protein RecA Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 379 amino acids
Theoretical weight: 41.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7G6 (Residues: 1-353; Coverage: 100%)
Gene names: JW2669, b2699, lexB, recA, recH, rnmB, tif, umuB, zab
Sequence domains:
LexA repressor Chains: I, J
Molecule details ›
Chains: I, J
Length: 205 amino acids
Theoretical weight: 22.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A7C2 (Residues: 1-202; Coverage: 100%)
Gene names: JW4003, b4043, exrA, lexA, spr, tsl, umuA
Sequence domains:
DNA (27-MER) Chain: L
Molecule details ›
Chain: L
Length: 27 nucleotides
Theoretical weight: 8.62 KDa

Ligands and Environments

2 bound ligands:
Ligand AGS 8 x AGS
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.93Å
Relevant EMDB volumes: EMD-41579
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

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