8trl

X-ray diffraction
2.4Å resolution

T cell recognition of citrullinated alpha-enolase peptide presented by HLA-DR4

Released:

Function and Biology Details

Reaction catalysed:
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-525853 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (7 distinct):
HLA class II histocompatibility antigen, DR alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 181 amino acids
Theoretical weight: 21.08 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01903 (Residues: 26-206; Coverage: 79%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
HLA class II histocompatibility antigen, DRB1 beta chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 190 amino acids
Theoretical weight: 22.28 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01911 (Residues: 30-219; Coverage: 80%)
Gene name: HLA-DRB1
Sequence domains:
Alpha-enolase Chains: C, F
Molecule details ›
Chains: C, F
Length: 13 amino acids
Theoretical weight: 1.42 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P06733 (Residues: 10-22; Coverage: 3%)
Gene names: ENO1, ENO1L1, MBPB1, MPB1
RA2.7 TCR alpha chain Chains: G, I
Molecule details ›
Chains: G, I
Length: 204 amino acids
Theoretical weight: 22.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
RA2.7 TCR beta chain Chains: H, J
Molecule details ›
Chains: H, J
Length: 245 amino acids
Theoretical weight: 28.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2
Unit cell:
a: 185.857Å b: 58.546Å c: 216.479Å
α: 90° β: 113.6° γ: 90°
R-values:
R R work R free
0.211 0.21 0.234
Expression systems:
  • Homo sapiens
  • Not provided
  • Escherichia coli BL21(DE3)