› 8tsr

Electron Microscopy
3.9Å resolution

Open, inward-facing MsbA structure (OIF4)

Released:
DOI: 10.2210/pdb8tsr/pdb
PDB entry 8tsr coloured by chain, front view.
PDB entry 8tsr coloured by chain, side view.
PDB entry 8tsr coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Native mass spectrometry and structural studies reveal modulation of MsbA-nucleotide interactions by lipids.
Zhang T, Lyu J, Yang B, Yun SD, Scott E, Zhao M, Laganowsky A
Nat Commun 15 5946 (2024)
PMID: 39009687
Related structures: EMD-41599

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + lipid A-core oligosaccharide(Side 1) = ADP + phosphate + lipid A-core oligosaccharide(Side 2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158081 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent lipid A-core flippase Chains: A, B
Molecule details ›
Chains: A, B
Length: 583 amino acids
Theoretical weight: 64.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60752 (Residues: 2-582; Coverage: 100%)
Gene names: JW0897, b0914, msbA
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-41599
Expression system: Escherichia coli

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