› 8ttv

X-ray diffraction
2Å resolution

Structure of SNX27 FERM complexed with Fam21A repeat 20 (1289-1302)

Released:
DOI: 10.2210/pdb8ttv/pdb
PDB entry 8ttv coloured by chain, front view.
PDB entry 8ttv coloured by chain, side view.
PDB entry 8ttv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for coupling of the WASH subunit FAM21 with the endosomal SNX27-Retromer complex.
Guo Q, Chen KE, Gimenez-Andres M, Jellett AP, Gao Y, Simonetti B, Liu M, Danson CM, Heesom KJ, Cullen PJ, Collins BM
Proc Natl Acad Sci U S A 121 e2405041121 (2024)
PMID: 39116126

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-563306 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sorting nexin-27 Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 30.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96L92 (Residues: 271-526; Coverage: 47%)
  • Best match: Q96L92-3 (Residues: 271-528)
Gene names: KIAA0488, My014, SNX27
Sequence domains:
WASH complex subunit 2A Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.56 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q641Q2 (Residues: 1289-1302; Coverage: 1%)
Gene names: FAM21A, FAM21B, WASHC2A

Ligands and Environments

1 bound ligand:
Ligand PEG 1 x PEG
1 modified residue:
Ligand CAF 2 x CAF

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 37.965Å b: 74.2Å c: 105.189Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.207 0.264
Expression systems:
  • Escherichia coli
  • Not provided

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