› 8tu9

Electron Microscopy
3.26Å resolution

Cryo-EM structure of HGSNAT-acetyl-CoA complex at pH 7.5

Released:
DOI: 10.2210/pdb8tu9/pdb
PDB entry 8tu9 coloured by chain, front view.
PDB entry 8tu9 coloured by chain, side view.
PDB entry 8tu9 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the human heparan-α-glucosaminide N-acetyltransferase (HGSNAT).
Navratna V, Kumar A, Rana JK, Mosalaganti S
Elife 13 (2024)
PMID: 39196614
Related structures: EMD-41620

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + heparan sulfate alpha-D-glucosaminide = CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-273728 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein; Heparan-alpha-glucosaminide N-acetyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 903 amino acids
Theoretical weight: 100.82 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
  • Canonical: Q68CP4 (Residues: 29-663; Coverage: 96%)
Gene names: GFP, HGSNAT, TMEM76
Sequence domains:

Ligands and Environments


Cofactor: Ligand ACO 2 x ACO
1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.26Å
Relevant EMDB volumes: EMD-41620
Expression system: Homo sapiens

Quick links

Citations

2 mentions without citation

Structural and mechanistic insights into a lysosomal membrane enzyme HGSNAT involved in Sanfilippo syndrome.
Zhao et al. (2024)
1 more