› 8ty0

X-ray diffraction
1.54Å resolution

Streptavidin variant S112E-K121H bound to bis-biotinylated Iron-porphyrin

Released:
DOI: 10.2210/pdb8ty0/pdb
Model geometry
Fit model/data
PDB entry 8ty0 coloured by chain, front view.
PDB entry 8ty0 coloured by chain, side view.
PDB entry 8ty0 coloured by chain, top view.
Source organism: Streptomyces avidinii
Entry authors: Follmer AH, Jakob RP, Mukherjee M, Ward TR

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 158 amino acids
Theoretical weight: 16.49 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22629 (Residues: 38-183; Coverage: 92%)
Sequence domains: Avidin family

Ligands and Environments

1 bound ligand:
Ligand SIK 2 x SIK
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 192.487Å b: 57.72Å c: 57.77Å
α: 90° β: 107.44° γ: 90°
R-values:
R R work R free
0.174 0.174 0.191
Expression system: Escherichia coli BL21(DE3)

Quick links

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO