› 8u0w

Electron Microscopy
2.99Å resolution

DdmD monomer in complex with ssDNA

Released:
DOI: 10.2210/pdb8u0w/pdb
PDB entry 8u0w coloured by chain, front view.
PDB entry 8u0w coloured by chain, side view.
PDB entry 8u0w coloured by chain, top view.
Source organisms:
Primary publication:
Plasmid targeting and destruction by the DdmDE bacterial defence system.
Bravo JPK, Ramos DA, Fregoso Ocampo R, Ingram C, Taylor DW
Nature (2024)
PMID: 38740055
Related structures: EMD-41790

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246792 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Helicase/UvrB N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 1220 amino acids
Theoretical weight: 140.01 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: B9TSM3 (Residues: 1-1220; Coverage: 100%)
Gene name: VPI2_0019c
DNA (5'-D(P*TP*TP*AP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.61 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.99Å
Relevant EMDB volumes: EMD-41790
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 citations in other articles

Defending against plasmids.
Du Toit A. (2024)