› 8u10

Electron Microscopy
3.2Å resolution

In situ cryo-EM structure of bacteriophage P22 gp1:gp4:gp5:gp10:gp9 N-term complex in conformation 1 at 3.2A resolution

Released:
DOI: 10.2210/pdb8u10/pdb
PDB entry 8u10 coloured by chain, front view.
PDB entry 8u10 coloured by chain, side view.
PDB entry 8u10 coloured by chain, top view.
Source organism: Salmonella phage P22
Primary publication:
Molecular architecture of Salmonella typhimurium virus P22 genome ejection machinery.
Iglesias SM, Lokareddy RK, Yang R, Li F, Yeggoni DP, David Hou CF, Leroux MN, Cortines JR, Leavitt JC, Bird M, Casjens SR, White S, Teschke CM, Cingolani G
J Mol Biol 168365 (2023)
PMID: 37952769
Related structures: EMD-41791

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 58-mer (preferred)
PDBe Complex ID:
PDB-CPX-536934 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Portal protein Chains: a, b, c, d, e, f, g, h, i, j, k, l
Molecule details ›
Chains: a, b, c, d, e, f, g, h, i, j, k, l
Length: 725 amino acids
Theoretical weight: 82.83 KDa
Source organism: Salmonella phage P22
UniProt:
  • Canonical: P26744 (Residues: 1-725; Coverage: 100%)
Gene name: 1
Sequence domains: Phage P22-like portal protein
Major capsid protein Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 430 amino acids
Theoretical weight: 46.8 KDa
Source organism: Salmonella phage P22
UniProt:
  • Canonical: P26747 (Residues: 1-430; Coverage: 100%)
Gene name: 5
Sequence domains: P22 coat protein - gene protein 5
Packaged DNA stabilization protein gp10 Chains: 1, 2, 3, 4, 5, 6
Molecule details ›
Chains: 1, 2, 3, 4, 5, 6
Length: 472 amino acids
Theoretical weight: 52.51 KDa
Source organism: Salmonella phage P22
UniProt:
  • Canonical: P26749 (Residues: 1-472; Coverage: 100%)
Gene name: 10
Sequence domains: Phage stabilisation protein
Tail spike protein Chains: 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 7, 8, 9
Molecule details ›
Chains: 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 7, 8, 9
Length: 667 amino acids
Theoretical weight: 71.92 KDa
Source organism: Salmonella phage P22
UniProt:
  • Canonical: P12528 (Residues: 1-667; Coverage: 100%)
Gene name: 9
Sequence domains:
Peptidoglycan hydrolase gp4 Chains: m, n, o, p, q, r, s, t, u, v, x, y
Molecule details ›
Chains: m, n, o, p, q, r, s, t, u, v, x, y
Length: 166 amino acids
Theoretical weight: 18.04 KDa
Source organism: Salmonella phage P22
UniProt:
  • Canonical: P26746 (Residues: 1-166; Coverage: 100%)
Gene name: 4
Sequence domains: P22 tail accessory factor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-41791

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