› 8u20

X-ray diffraction
1.9Å resolution

A Highly Stable Variant of Corynactis Californica Green Fluorescent Protein, ccGFP 5

Released:
DOI: 10.2210/pdb8u20/pdb
PDB entry 8u20 coloured by chain, front view.
PDB entry 8u20 coloured by chain, side view.
PDB entry 8u20 coloured by chain, top view.
Source organism: Corynactis californica
Primary publication:
Engineering Highly Stable Variants of Corynactis Californica Green Fluorescent Proteins.
Hung LW, Terwilliger TC, Waldo GS, Nguyen HB
OpenAccess logo Protein Sci e4886 (2023)
PMID: 38151801

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274600 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GFP-type green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 25.9 KDa
Source organism: Corynactis californica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1ALD4 (Residues: 1-220; Coverage: 100%)
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand C12 2 x C12

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 50.357Å b: 87.588Å c: 147.657Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.2
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Engineering highly stable variants of Corynactis californica green fluorescent proteins.
Hung et al. (2024)