› 8u45

X-ray diffraction
2.1Å resolution

Crystal Structure Analysis of Aspergillus fumigatus alkaline protease

Released:
DOI: 10.2210/pdb8u45/pdb
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PDB entry 8u45 coloured by chain, front view.
PDB entry 8u45 coloured by chain, side view.
PDB entry 8u45 coloured by chain, top view.
Source organism: Aspergillus fumigatus Af293
Primary publication:
Targeting Aspergillus allergen oryzin with a chemical probe at atomic precision.
Pattelli ON, Diec DDL, Guo W, Russi S, Fernandez D
OpenAccess logo Sci Rep 13 17926 (2023)
PMID: 37864071

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyze peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-273479 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alkaline protease 1 Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 11.42 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Escherichia coli
UniProt:
  • Canonical: P28296 (Residues: 21-121; Coverage: 26%)
Gene names: AFUA_4G11800, alk1, alp, alp1
Sequence domains: Peptidase inhibitor I9
Alkaline protease 1 Chain: C
Molecule details ›
Chain: C
Length: 282 amino acids
Theoretical weight: 28.73 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Escherichia coli
UniProt:
  • Canonical: P28296 (Residues: 122-403; Coverage: 74%)
Gene names: AFUA_4G11800, alk1, alp, alp1
Sequence domains: Subtilase family

Ligands and Environments

3 bound ligands:
Ligand PEG 6 x PEG
Ligand CA 1 x CA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P21
Unit cell:
a: 42.91Å b: 94.592Å c: 52.925Å
α: 90° β: 103.2° γ: 90°
R-values:
R R work R free
0.193 0.191 0.23
Expression system: Escherichia coli

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