› 8u4n

Electron Microscopy
2.72Å resolution

Structure of Apo CXCR4/Gi complex

Released:
DOI: 10.2210/pdb8u4n/pdb
PDB entry 8u4n coloured by chain, front view.
PDB entry 8u4n coloured by chain, side view.
PDB entry 8u4n coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into CXCR4 modulation and oligomerization.
Saotome K, McGoldrick LL, Ho JH, Ramlall TF, Shah S, Moore MJ, Kim JH, Leidich R, Olson WC, Franklin MC
Nat Struct Mol Biol (2024)
PMID: 39313635
Related structures: EMD-41888

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-273725 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 365 amino acids
Theoretical weight: 41.59 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 2-354; Coverage: 100%)
  • Best match: P63096-2 (Residues: 1-302)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.53 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
C-X-C chemokine receptor type 4; Green fluorescent protein Chain: R
Molecule details ›
Chain: R
Length: 632 amino acids
Theoretical weight: 71.06 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P61073 (Residues: 2-352; Coverage: 100%)
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene names: CXCR4, GFP
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.72Å
Relevant EMDB volumes: EMD-41888
Expression system: Spodoptera frugiperda

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