› 8u4v

Electron Microscopy
2.99Å resolution

Cryo-EM structure of human claudin-4 complex with Clostridium perfringens enterotoxin C-terminal domain, sFab COP-1, and Nanobody

Released:
DOI: 10.2210/pdb8u4v/pdb
PDB entry 8u4v coloured by chain, front view.
PDB entry 8u4v coloured by chain, side view.
PDB entry 8u4v coloured by chain, top view.
Source organisms:
Primary publication:
Structural and biophysical insights into targeting of claudin-4 by a synthetic antibody fragment.
Erramilli SK, Dominik PK, Ogbu CP, Kossiakoff AA, Vecchio AJ
OpenAccess logo Commun Biol 7 733 (2024)
PMID: 38886509
Related structures: EMD-41899

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-233150 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Claudin-4 Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 22.61 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Heat-labile enterotoxin B chain Chain: B
Molecule details ›
Chain: B
Length: 125 amino acids
Theoretical weight: 14.04 KDa
Source organism: Clostridium perfringens
Expression system: Trichoplusia ni
COP-1 sFab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
COP-1 sFab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 261 amino acids
Theoretical weight: 28.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Anti-fab nanobody Chain: K
Molecule details ›
Chain: K
Length: 121 amino acids
Theoretical weight: 13.18 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand AV0 1 x AV0
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.99Å
Relevant EMDB volumes: EMD-41899
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

1 review citation

Expression and Targeted Application of Claudins Family in Hepatobiliary and Pancreatic Diseases.
Du et al. (2024)

1 mention without citation

Computational design of soluble and functional membrane protein analogues.
Goverde et al. (2024)