› 8u5o

X-ray diffraction
1.5Å resolution

The structure of the catalytic domain of NanI sialdase in complex with Neu5Gc

Released:
DOI: 10.2210/pdb8u5o/pdb
PDB entry 8u5o coloured by chain, front view.
PDB entry 8u5o coloured by chain, side view.
PDB entry 8u5o coloured by chain, top view.
Source organism: Clostridium perfringens ATCC 13124
Primary publication:
A "terminal" case of glycan catabolism: structural and enzymatic characterization of the sialidases of Clostridium perfringens.
Medley BJ, Low KE, Irungu JDW, Kipchumba L, Daneshgar P, Liu L, Garber JM, Klassen L, Inglis GD, Boons GJ, Zandberg WF, Abbott DW, Boraston AB
J Biol Chem 107750 (2024)
PMID: 39251137

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-251048 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exo-alpha-sialidase Chain: A
Molecule details ›
Chain: A
Length: 449 amino acids
Theoretical weight: 50.19 KDa
Source organism: Clostridium perfringens ATCC 13124
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

3 bound ligands:
Ligand NGC 1 x NGC
Ligand CA 2 x CA
Ligand NO3 7 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-002
Spacegroup: P212121
Unit cell:
a: 69.27Å b: 72.305Å c: 97.137Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.154 0.178
Expression system: Escherichia coli BL21(DE3)

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