› 8u65

Electron Microscopy
3.01Å resolution

Cryo-EM structure of PsBphP in Pfr state, splayed PSM only

Released:
DOI: 10.2210/pdb8u65/pdb
PDB entry 8u65 coloured by chain, front view.
PDB entry 8u65 coloured by chain, side view.
PDB entry 8u65 coloured by chain, top view.
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Primary publication:
Signaling by a bacterial phytochrome histidine kinase involves a conformational cascade reorganizing the dimeric photoreceptor.
Burgie ES, Basore K, Rau MJ, Summers B, Mickles AJ, Grigura V, Fitzpatrick JAJ, Vierstra RD
Nat Commun 15 6853 (2024)
PMID: 39127720
Related structures: EMD-41944

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-275054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 746 amino acids
Theoretical weight: 82.38 KDa
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q885D3 (Residues: 1-745; Coverage: 100%)
Gene names: PSPTO_1902, bphP
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand LBV 2 x LBV
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.01Å
Relevant EMDB volumes: EMD-41944
Expression system: Escherichia coli BL21(DE3)

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Citations

1 mention without citation

Signaling by a bacterial phytochrome histidine kinase involves a conformational cascade reorganizing the dimeric photoreceptor.
Burgie et al. (2024)