› 8u6y

Electron Microscopy
2.8Å resolution

Preholo-Proteasome from Beta 3 D205 deletion

Released:
DOI: 10.2210/pdb8u6y/pdb
PDB entry 8u6y coloured by chain, front view.
PDB entry 8u6y coloured by chain, side view.
PDB entry 8u6y coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Mechanism of autocatalytic activation during proteasome assembly.
Velez B, Walsh RM, Rawson S, Razi A, Adams L, Perez EF, Jiao F, Blickling M, Rajakumar T, Fung D, Huang L, Hanna J
Nat Struct Mol Biol (2024)
PMID: 38600323
Related structures: EMD-41963

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 34-mer (preferred)
PDBe Complex ID:
PDB-CPX-228101 (preferred)
Entry contents:
17 distinct polypeptide molecules
Macromolecules (17 distinct):
Proteasome subunit alpha type-1 Chains: A, R
Molecule details ›
Chains: A, R
Length: 252 amino acids
Theoretical weight: 28.03 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P21243 (Residues: 1-252; Coverage: 100%)
Gene names: PRC2, PRS2, SCL1, YGL011C
Sequence domains:
Proteasome subunit alpha type-2 Chains: B, S
Molecule details ›
Chains: B, S
Length: 250 amino acids
Theoretical weight: 27.19 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P23639 (Residues: 1-250; Coverage: 100%)
Gene names: PRE8, PRS4, YML092C
Sequence domains:
Proteasome subunit alpha type-3 Chains: C, T
Molecule details ›
Chains: C, T
Length: 245 amino acids
Theoretical weight: 27.18 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P23638 (Residues: 1-245; Coverage: 95%)
Gene names: PRE9, PRS5, YGR135W
Sequence domains:
Proteasome subunit alpha type-4 Chains: D, U
Molecule details ›
Chains: D, U
Length: 254 amino acids
Theoretical weight: 28.48 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40303 (Residues: 1-254; Coverage: 100%)
Gene names: PRE6, YOL038W
Sequence domains:
Proteasome subunit alpha type-5 Chains: E, V
Molecule details ›
Chains: E, V
Length: 260 amino acids
Theoretical weight: 28.65 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P32379 (Residues: 1-260; Coverage: 100%)
Gene names: DOA5, G9155, PUP2, YGR253C
Sequence domains:
Proteasome subunit alpha type-6 Chains: F, W
Molecule details ›
Chains: F, W
Length: 234 amino acids
Theoretical weight: 25.63 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40302 (Residues: 1-234; Coverage: 100%)
Gene names: PRE5, YM9924.06, YMR314W
Sequence domains:
Probable proteasome subunit alpha type-7 Chains: G, X
Molecule details ›
Chains: G, X
Length: 288 amino acids
Theoretical weight: 31.58 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P21242 (Residues: 1-288; Coverage: 100%)
Gene names: O6650, PRC1, PRE10, PRS1, YOR362C
Sequence domains:
Proteasome maturation factor UMP1 Chains: H, Y
Molecule details ›
Chains: H, Y
Length: 148 amino acids
Theoretical weight: 16.78 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P38293 (Residues: 1-148; Coverage: 100%)
Gene names: UMP1, YBR1234, YBR173C
Sequence domains: Proteasome maturation factor UMP1
Proteasome subunit beta type-2 Chains: I, Z
Molecule details ›
Chains: I, Z
Length: 261 amino acids
Theoretical weight: 28.3 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P25043 (Residues: 1-261; Coverage: 100%)
Gene names: PUP1, YOR157C
Sequence domains:
Proteasome subunit beta type-3 Chains: J, a
Molecule details ›
Chains: J, a
Length: 204 amino acids
Theoretical weight: 22.51 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P25451 (Residues: 1-204; Coverage: 100%)
Gene names: PUP3, YER094C
Sequence domains: Proteasome subunit
Proteasome subunit beta type-4 Chains: K, b
Molecule details ›
Chains: K, b
Length: 198 amino acids
Theoretical weight: 22.55 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P22141 (Residues: 1-198; Coverage: 100%)
Gene names: PRE1, YER012W
Sequence domains: Proteasome subunit
Proteasome subunit beta type-5 Chains: L, c
Molecule details ›
Chains: L, c
Length: 287 amino acids
Theoretical weight: 31.67 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P30656 (Residues: 1-287; Coverage: 100%)
Gene names: DOA3, P8283.10, PRE2, PRG1, YPR103W
Sequence domains: Proteasome subunit
Proteasome subunit beta type-6 Chains: M, d
Molecule details ›
Chains: M, d
Length: 241 amino acids
Theoretical weight: 26.91 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P23724 (Residues: 1-241; Coverage: 100%)
Gene names: PRE7, PRS3, PTS1, YBL0407, YBL041W
Sequence domains: Proteasome subunit
Proteasome subunit beta type-1 Chains: N, e
Molecule details ›
Chains: N, e
Length: 215 amino acids
Theoretical weight: 23.57 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P38624 (Residues: 1-215; Coverage: 100%)
Gene names: J1407, PRE3, YJL001W
Sequence domains: Proteasome subunit
Proteasome chaperone 1 Chains: O, f
Molecule details ›
Chains: O, f
Length: 276 amino acids
Theoretical weight: 30.72 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q05778 (Residues: 1-276; Coverage: 100%)
Gene names: PBA1, POC1, YLR199C
Sequence domains: POC1 chaperone
Proteasome assembly chaperone 2 Chains: P, g
Molecule details ›
Chains: P, g
Length: 267 amino acids
Theoretical weight: 30.76 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P36040 (Residues: 1-267; Coverage: 100%)
Gene names: ADD66, PBA2, POC2, YKL206C
Sequence domains: PAC2 family
Proteasome subunit beta type-7 Chains: Q, h
Molecule details ›
Chains: Q, h
Length: 266 amino acids
Theoretical weight: 29.47 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P30657 (Residues: 1-266; Coverage: 100%)
Gene names: PRE4, YFR050C
Sequence domains: Proteasome subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-41963

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