› 8u7b

X-ray diffraction
2.66Å resolution

Crystal structure of Apo form of Short Prokaryotic Argonaute TIR-APAZ (SPARTA) heterodimer

Released:
DOI: 10.2210/pdb8u7b/pdb
PDB entry 8u7b coloured by chain, front view.
PDB entry 8u7b coloured by chain, side view.
PDB entry 8u7b coloured by chain, top view.
Source organism: Thermoflavifilum thermophilum
Primary publication:
Nucleic acid mediated activation of a short prokaryotic Argonaute immune system.
Kottur J, Malik R, Aggarwal AK
Nat Commun 15 4852 (2024)
PMID: 38844755

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232317 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
TIR domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 449 amino acids
Theoretical weight: 53.14 KDa
Source organism: Thermoflavifilum thermophilum
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1I7NFG5 (Residues: 1-450; Coverage: 100%)
Gene name: SAMN05660895_1670
Sequence domains: TIR domain
Piwi domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 507 amino acids
Theoretical weight: 58.3 KDa
Source organism: Thermoflavifilum thermophilum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1I7NFD7 (Residues: 1-507; Coverage: 100%)
Gene name: SAMN05660895_1671

Ligands and Environments

2 bound ligands:
Ligand PRO 7 x PRO
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-2
Spacegroup: P6522
Unit cell:
a: 197.181Å b: 197.181Å c: 183.407Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.179 0.239
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

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