› 8u7q

Electron Microscopy
3.3Å resolution

Human retinal variant phosphomimetic IMPDH1(546)-S477D filament bound by GTP, ATP, IMP, and NAD+, octamer-centered

Released:
DOI: 10.2210/pdb8u7q/pdb
PDB entry 8u7q coloured by chain, front view.
PDB entry 8u7q coloured by chain, side view.
PDB entry 8u7q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Light-sensitive phosphorylation regulates retinal IMPDH1 activity and filament assembly.
Calise SJ, O'Neill AG, Burrell AL, Dickinson MS, Molfino J, Clarke C, Quispe J, Sokolov D, Buey RM, Kollman JM
J Cell Biol 223 (2024)
PMID: 38323936
Related structures: EMD-41989

Function and Biology Details

Reaction catalysed: 
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149076 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine-5'-monophosphate dehydrogenase 1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 548 amino acids
Theoretical weight: 58.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20839 (Residues: 1-509; Coverage: 99%)
Gene names: IMPD1, IMPDH1
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
3 bound ligands:
Ligand GTP 16 x GTP
Ligand IMP 8 x IMP
Ligand ATP 8 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-41989
Expression system: Escherichia coli BL21(DE3)

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