› 8u82

Electron Microscopy
3.84Å resolution

KCTD5/Cullin3/Gbeta1gamma2 Complex: State B From Composite RELION Multi-body Refinement Map

Released:
DOI: 10.2210/pdb8u82/pdb
PDB entry 8u82 coloured by chain, front view.
PDB entry 8u82 coloured by chain, side view.
PDB entry 8u82 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and dynamics of a pentameric KCTD5/CUL3/Gβγ E3 ubiquitin ligase complex.
Nguyen DM, Rath DH, Devost D, Pétrin D, Rizk R, Ji AX, Narayanan N, Yong D, Zhai A, Kuntz DA, Mian MUQ, Pomroy NC, Keszei AFA, Benlekbir S, Mazhab-Jafari MT, Rubinstein JL, Hébert TE, Privé GG
Proc Natl Acad Sci U S A 121 e2315018121 (2024)
PMID: 38625940
Related structures: EMD-42000

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
PDBe Complex ID:
PDB-CPX-274016 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chains: B1, B2, B3, B4, B5
Molecule details ›
Chains: B1, B2, B3, B4, B5
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Cullin-3 Chains: C1, C2, C3, C4, C5
Molecule details ›
Chains: C1, C2, C3, C4, C5
Length: 380 amino acids
Theoretical weight: 44.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13618 (Residues: 2-381; Coverage: 50%)
Gene names: CUL3, KIAA0617
Sequence domains: Cullin family
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chains: G1, G2, G3, G4, G5
Molecule details ›
Chains: G1, G2, G3, G4, G5
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
BTB/POZ domain-containing protein KCTD5 Chains: K1, K2, K3, K4, K5
Molecule details ›
Chains: K1, K2, K3, K4, K5
Length: 234 amino acids
Theoretical weight: 26.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NXV2 (Residues: 1-234; Coverage: 100%)
Gene name: KCTD5
Sequence domains: BTB/POZ domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.84Å
Relevant EMDB volumes: EMD-42000
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)

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