› 8u8f

Electron Microscopy
3.49Å resolution

GPR3 Orphan G-coupled Protein Receptor in complex with Dominant Negative Gs.

Released:
DOI: 10.2210/pdb8u8f/pdb
PDB entry 8u8f coloured by chain, front view.
PDB entry 8u8f coloured by chain, side view.
PDB entry 8u8f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Lipid-Dependent Activation of the Orphan G Protein-Coupled Receptor, GPR3.
Russell IC, Zhang X, Bumbak F, McNeill SM, Josephs TM, Leeming MG, Christopoulos G, Venugopal H, Flocco MM, Sexton PM, Wootten D, Belousoff MJ
Biochemistry (2024)
PMID: 38376112
Related structures: EMD-42023

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-545786 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.7 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.41 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 58 amino acids
Theoretical weight: 6.38 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 5-62; Coverage: 82%)
Gene name: GNG2
Sequence domains: GGL domain
G-protein coupled receptor 3 Chain: R
Molecule details ›
Chain: R
Length: 390 amino acids
Theoretical weight: 41.79 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P46089 (Residues: 2-330; Coverage: 100%)
Gene names: ACCA, GPR3
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

1 bound ligand:
Ligand PLM 1 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.49Å
Relevant EMDB volumes: EMD-42023
Expression system: Trichoplusia ni

Quick links

Citations

1 citations in other articles

Shifting our perspective on orphan G protein-coupled receptors.
Smith et al. (2024)