› 8u90

X-ray diffraction
1.85Å resolution

Crystal structure of N-acetylneuraminate lyase (NanA) from Klebsiella aerogenes (Apo, hexagonal form)

Released:
DOI: 10.2210/pdb8u90/pdb
PDB entry 8u90 coloured by chain, front view.
PDB entry 8u90 coloured by chain, side view.
PDB entry 8u90 coloured by chain, top view.
Source organism: Klebsiella aerogenes KCTC 2190
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
Aceneneuramate = N-acetyl-D-mannosamine + pyruvate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229606 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylneuraminate lyase Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 33.67 KDa
Source organism: Klebsiella aerogenes KCTC 2190
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3FJT8 (Residues: 1-297; Coverage: 100%)
Gene names: EAE_03255, nanA
Sequence domains: Dihydrodipicolinate synthetase family

Ligands and Environments

4 bound ligands:
Ligand NA 1 x NA
Ligand CL 3 x CL
Ligand IOD 2 x IOD
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 19-ID
Spacegroup: P6222
Unit cell:
a: 97.599Å b: 97.599Å c: 204.905Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.171 0.199
Expression system: Escherichia coli BL21(DE3)

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