› 8uce

X-ray diffraction
2.12Å resolution

Thermophilic RNA Ligase from Palaeococcus pacificus + AMP

Released:
DOI: 10.2210/pdb8uce/pdb
PDB entry 8uce coloured by chain, front view.
PDB entry 8uce coloured by chain, side view.
PDB entry 8uce coloured by chain, top view.
Source organism: Palaeococcus pacificus DY20341
Primary publication:
Characterisation and engineering of a thermophilic RNA ligase from Palaeococcus pacificus.
Rousseau M, Oulavallickal T, Williamson A, Arcus V, Patrick WM, Hicks J
OpenAccess logo Nucleic Acids Res (2024)
PMID: 38421610

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239984 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP dependent DNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 402 amino acids
Theoretical weight: 46.97 KDa
Source organism: Palaeococcus pacificus DY20341
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A075LQ94 (Residues: 1-381; Coverage: 100%)
Gene name: PAP_02190
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand AMP 2 x AMP
Ligand GOL 7 x GOL
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Unit cell:
a: 81.216Å b: 98.799Å c: 142.977Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.237
Expression system: Escherichia coli BL21(DE3)

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