› 8ucw

Electron Microscopy
3.64Å resolution

Complete DNA termination subcomplex 2 of Xenopus laevis DNA polymerase alpha-primase

Released:
DOI: 10.2210/pdb8ucw/pdb
PDB entry 8ucw coloured by chain, front view.
PDB entry 8ucw coloured by chain, side view.
PDB entry 8ucw coloured by chain, top view.
Source organisms:
Primary publication:
A mechanistic model of primer synthesis from catalytic structures of DNA polymerase α-primase.
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024)
PMID: 38491139
Related structures: EMD-42142

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231615 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (3 distinct):
DNA polymerase alpha catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 1127 amino acids
Theoretical weight: 129.01 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9DE46 (Residues: 335-1458; Coverage: 77%)
Gene names: pola, pola1
Sequence domains:
DNA template Chain: C
Molecule details ›
Chain: C
Length: 59 nucleotides
Theoretical weight: 18.2 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA-DNA/DNA primer Chain: D
Molecule details ›
Chain: D
Length: 29 nucleotides
Theoretical weight: 9.25 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand DGT 1 x DGT
Ligand MG 2 x MG
2 modified residues:
Ligand GTP 1 x GTP
Ligand DOC 1 x DOC

Experiments and Validation Details

Entry percentile scores
Resolution: 3.64Å
Relevant EMDB volumes: EMD-42142
Expression systems:
  • Spodoptera frugiperda
  • Not provided

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