› 8ucx

X-ray diffraction
1.57Å resolution

Dihydrofolate Reductase Complexed with Folate

Released:
DOI: 10.2210/pdb8ucx/pdb
Model geometry
Fit model/data
PDB entry 8ucx coloured by chain, front view.
PDB entry 8ucx coloured by chain, side view.
PDB entry 8ucx coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Fried SDE, Boxer SG

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142189 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABQ4 (Residues: 1-159; Coverage: 100%)
Gene names: JW0047, b0048, folA, tmrA
Sequence domains: Dihydrofolate reductase

Ligands and Environments

2 bound ligands:
Ligand FOL 1 x FOL
Ligand MN 3 x MN
1 modified residue:
Ligand OCS 1 x OCS

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-1
Spacegroup: I4
Unit cell:
a: 104.4Å b: 104.4Å c: 33.908Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.217
Expression system: Escherichia coli BL21(DE3)

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