› 8uf7

Electron Microscopy
3.2Å resolution

Cryo-EM structure of POmAb, a Type-I anti-prothrombin antiphospholipid antibody, bound to kringle-1 of human prothrombin

Released:
DOI: 10.2210/pdb8uf7/pdb
PDB entry 8uf7 coloured by chain, front view.
PDB entry 8uf7 coloured by chain, side view.
PDB entry 8uf7 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure and functional basis of prothrombin recognition by a type I antiprothrombin antiphospholipid antibody.
Kumar S, Summers B, Basore K, Pengo V, Flaumenhaft R, Pozzi N
Blood 143 2005-2011 (2024)
PMID: 38437497
Related structures: EMD-42185

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239904 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
POmAb Light Chain Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 23.56 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
POmAb Heavy Chain Chain: B
Molecule details ›
Chain: B
Length: 218 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Prothrombin Chain: C
Molecule details ›
Chain: C
Length: 579 amino acids
Theoretical weight: 65.39 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00734 (Residues: 44-622; Coverage: 97%)
Gene name: F2
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-42185
Expression system: Cricetulus griseus

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