› 8uh3

Electron Microscopy
3.31Å resolution

Serotonin 1E receptor (5-HT1eR)-Gi1 Complex bound with Setiptiline

Released:
DOI: 10.2210/pdb8uh3/pdb
PDB entry 8uh3 coloured by chain, front view.
PDB entry 8uh3 coloured by chain, side view.
PDB entry 8uh3 coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the unexpected agonism of tetracyclic antidepressants at serotonin receptors 5-HT1eR and 5-HT1FR.
Zilberg G, Parpounas AK, Warren AL, Fiorillo B, Provasi D, Filizola M, Wacker D
OpenAccess logo Sci Adv 10 eadk4855 (2024)
PMID: 38630816
Related structures: EMD-42245

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-243752 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 358 amino acids
Theoretical weight: 39.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
5-hydroxytryptamine receptor 1E Chain: D
Molecule details ›
Chain: D
Length: 365 amino acids
Theoretical weight: 41.8 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P28566 (Residues: 1-365; Coverage: 100%)
Gene name: HTR1E
Sequence domains: 7 transmembrane receptor (rhodopsin family)
ScFv16 Chain: E
Molecule details ›
Chain: E
Length: 259 amino acids
Theoretical weight: 27.78 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

2 bound ligands:
Ligand CLR 4 x CLR
Ligand WOQ 1 x WOQ
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.31Å
Relevant EMDB volumes: EMD-42245
Expression system: Spodoptera frugiperda

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