› 8ui8

Electron Microscopy
3.1Å resolution

Cryo-EM map of human clamp-clamp loader ATAD5-RFC-two PCNAs complex in intermediate state 3

Released:
DOI: 10.2210/pdb8ui8/pdb
PDB entry 8ui8 coloured by chain, front view.
PDB entry 8ui8 coloured by chain, side view.
PDB entry 8ui8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The human ATAD5 has evolved unique structural elements to function exclusively as a PCNA unloader.
Wang F, He Q, Yao NY, O'Donnell ME, Li H
Nat Struct Mol Biol (2024)
PMID: 38871854
Related structures: EMD-42288

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-244796 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
ATPase family AAA domain-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 1032 amino acids
Theoretical weight: 116.42 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q96QE3 (Residues: 813-1844; Coverage: 56%)
Gene names: ATAD5, C17orf41, Elg1, FRAG1
Sequence domains: ATPase family associated with various cellular activities (AAA)
Replication factor C subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 354 amino acids
Theoretical weight: 39.2 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P35250 (Residues: 1-354; Coverage: 100%)
Gene name: RFC2
Sequence domains:
Replication factor C subunit 5 Chain: C
Molecule details ›
Chain: C
Length: 340 amino acids
Theoretical weight: 38.55 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P40937 (Residues: 1-340; Coverage: 100%)
Gene name: RFC5
Sequence domains:
Replication factor C subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 363 amino acids
Theoretical weight: 39.74 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P35249 (Residues: 1-363; Coverage: 100%)
Gene name: RFC4
Sequence domains:
Replication factor C subunit 3 Chain: E
Molecule details ›
Chain: E
Length: 356 amino acids
Theoretical weight: 40.61 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P40938 (Residues: 1-356; Coverage: 100%)
Gene name: RFC3
Sequence domains:
Proliferating cell nuclear antigen Chains: F, G
Molecule details ›
Chains: F, G
Length: 261 amino acids
Theoretical weight: 28.8 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand MG 3 x MG
Ligand AGS 3 x AGS
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-42288
Expression system: Insect cell expression vector pTIE1

Quick links