› 8ukz

X-ray diffraction
1.95Å resolution

Structure of P450Blt from Micromonospora sp. MW-13 with E238A Mutation

Released:
DOI: 10.2210/pdb8ukz/pdb
PDB entry 8ukz coloured by chain, front view.
PDB entry 8ukz coloured by chain, side view.
PDB entry 8ukz coloured by chain, top view.
Source organism: Micromonospora sp. MW-13
Primary publication:
Structural Insights into a Side Chain Cross-Linking Biarylitide P450 from RiPP Biosynthesis
Hansen MH, Keto A, Treisman M, Sasi VM, Coe L, Zhao Y, Padva L, Hess C, Leichthammer V, Machell DL, Schittenhelm RB, Jackson CJ, Tailhades J, Crusemann M, De Voss JJ, Krenske EH, Cryle MJ
ACS Catalysis 812-826 (2024)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238481 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450-SU1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 420 amino acids
Theoretical weight: 45.27 KDa
Source organism: Micromonospora sp. MW-13
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A3E2YLT4 (Residues: 1-399; Coverage: 100%)
Gene name: C5N14_21030
Sequence domains: Cytochrome P450

Ligands and Environments

4 bound ligands:
Ligand HEM 3 x HEM
Ligand TFA 4 x TFA
Ligand ACT 4 x ACT
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 61.957Å b: 94.48Å c: 105.985Å
α: 90° β: 92.68° γ: 90°
R-values:
R R work R free
0.206 0.205 0.223
Expression system: Escherichia coli BL21(DE3)

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