› 8umw

Electron Microscopy
2.93Å resolution

Atomic model of the human CTF18-RFC-PCNA-DNA ternary complex in the five-subunit binding state (state 4)

Released:
DOI: 10.2210/pdb8umw/pdb
PDB entry 8umw coloured by chain, front view.
PDB entry 8umw coloured by chain, side view.
PDB entry 8umw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM reveals a nearly complete PCNA loading process and unique features of the human alternative clamp loader CTF18-RFC.
He Q, Wang F, O'Donnell ME, Li H
OpenAccess logo Proc Natl Acad Sci U S A 121 e2319727121 (2024)
PMID: 38669181
Related structures: EMD-42386

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270846 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (8 distinct):
Chromosome transmission fidelity protein 18 homolog Chain: A
Molecule details ›
Chain: A
Length: 975 amino acids
Theoretical weight: 107.52 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q8WVB6 (Residues: 1-975; Coverage: 100%)
Gene names: C16orf41, CHTF18, CTF18
Sequence domains: ATPase family associated with various cellular activities (AAA)
Replication factor C subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 354 amino acids
Theoretical weight: 39.2 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P35250 (Residues: 1-354; Coverage: 100%)
Gene name: RFC2
Sequence domains:
Replication factor C subunit 5 Chain: C
Molecule details ›
Chain: C
Length: 340 amino acids
Theoretical weight: 38.55 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P40937 (Residues: 1-340; Coverage: 100%)
Gene name: RFC5
Sequence domains:
Replication factor C subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 363 amino acids
Theoretical weight: 39.74 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P35249 (Residues: 1-363; Coverage: 100%)
Gene name: RFC4
Sequence domains:
Replication factor C subunit 3 Chain: E
Molecule details ›
Chain: E
Length: 356 amino acids
Theoretical weight: 40.61 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P40938 (Residues: 1-356; Coverage: 100%)
Gene name: RFC3
Sequence domains:
Proliferating cell nuclear antigen Chains: F, G, H
Molecule details ›
Chains: F, G, H
Length: 261 amino acids
Theoretical weight: 28.8 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
DNA (40-MER) Chain: I
Molecule details ›
Chain: I
Length: 40 nucleotides
Theoretical weight: 12.21 KDa
DNA (20-MER) Chain: J
Molecule details ›
Chain: J
Length: 20 nucleotides
Theoretical weight: 6.14 KDa

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand AGS 4 x AGS
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.93Å
Relevant EMDB volumes: EMD-42386
Expression system: Insect cell expression vector pTIE1

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