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Electron Microscopy
2.91Å resolution

Structure of the KEOPS complex (Cgi121/Bud32/Kae1/Pcc1)

Released:

Function and Biology Details

Reactions catalysed:
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-284005 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein Chain: B
Molecule details ›
Chain: B
Length: 203 amino acids
Theoretical weight: 23.96 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q58530 (Residues: 333-535; Coverage: 38%)
Gene name: MJ1130
Sequence domains: RIO1 family
Regulatory protein Cgi121 Chain: C
Molecule details ›
Chain: C
Length: 146 amino acids
Theoretical weight: 16.72 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57646 (Residues: 1-145; Coverage: 100%)
Gene names: MJ0187, cgi121
Sequence domains: Kinase binding protein CGI-121
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein Chain: K
KEOPS complex subunit Pcc1 Chain: P

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.91Å
Relevant EMDB volumes: EMD-42407
Expression system: Escherichia coli BL21(DE3)