› 8uoh

X-ray diffraction
2.15Å resolution

Crystal structure of human NUAK1-MARK3 kinase domain chimera bound with small molecule inhibitor #10

Released:
DOI: 10.2210/pdb8uoh/pdb
PDB entry 8uoh coloured by chain, front view.
PDB entry 8uoh coloured by chain, side view.
PDB entry 8uoh coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Delker SL, Abendroth J

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151008 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MAP/microtubule affinity-regulating kinase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 37.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli B
UniProt:
  • Canonical: P27448 (Residues: 48-370; Coverage: 43%)
Gene names: CTAK1, EMK2, MARK3
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand EDO 9 x EDO
Ligand NI 1 x NI
Ligand X4W 1 x X4W
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 61.88Å b: 113.41Å c: 116.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.22
Expression system: Escherichia coli B

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