8uoq

Electron Microscopy
3.8Å resolution

Composite map of PIC_delta_TFIIK form2

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 30-mer (preferred)
PDBe Complex ID:
PDB-CPX-279220 (preferred)
Entry contents:
28 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (30 distinct):
Transcription initiation factor IIB Chain: M
Molecule details ›
Chain: M
Length: 345 amino acids
Theoretical weight: 38.26 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P29055 (Residues: 1-345; Coverage: 100%)
Gene names: P9513.4, SUA7, YPR086W
Sequence domains:
DNA-directed RNA polymerase II subunit RPB1 Chain: A
DNA-directed RNA polymerase II subunit RPB2 Chain: B
DNA-directed RNA polymerase II subunit RPB3 Chain: C
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chain: E
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: F
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
DNA-directed RNA polymerase II subunit RPB9 Chain: I
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
DNA-directed RNA polymerase II subunit RPB11 Chain: K
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chain: L
Transcription initiation factor IIF subunit alpha Chain: Q
Transcription initiation factor IIF subunit beta Chain: P
Transcription elongation factor S-II Chain: S
TATA-box-binding protein Chain: O
Transcription initiation factor IIA large subunit Chain: U
Transcription initiation factor IIA subunit 2 Chain: V
Transcription initiation factor IIE subunit alpha Chain: W
Transcription initiation factor IIE subunit beta Chain: X
DNA-directed RNA polymerase II subunit RPB4 Chain: D
DNA-directed RNA polymerase II subunit RPB7 Chain: G
General transcription and DNA repair factor IIH helicase subunit XPD/RAD3 Chain: 0
General transcription and DNA repair factor IIH subunit TFB1 Chain: 1
General transcription and DNA repair factor IIH subunit TFB4 Chain: 4
General transcription and DNA repair factor IIH subunit SSL1 Chain: 6
General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2 Chain: 7
General transcription and DNA repair factor IIH subunit TFB2 Chain: 2
General transcription and DNA repair factor IIH subunit TFB5 Chain: 5
non-template strand Chain: N
template strand Chain: T

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-42437
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'