› 8uox

Electron Microscopy
4.6Å resolution

Cryo-EM structure of a Counterclockwise locked form of the Salmonella enterica Typhimurium flagellar C-ring, with C34 symmetry applied

Released:
DOI: 10.2210/pdb8uox/pdb
PDB entry 8uox coloured by chain, front view.
PDB entry 8uox coloured by chain, side view.
PDB entry 8uox coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Structural basis of directional switching by the bacterial flagellum.
Johnson S, Deme JC, Furlong EJ, Caesar JJE, Chevance FFV, Hughes KT, Lea SM
Nat Microbiol (2024)
PMID: 38459206
Related structures: EMD-42439

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 204-mer (preferred)
PDBe Complex ID:
PDB-CPX-272531 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Flagellar M-ring protein Chains: A1, A2, A3, A4, A5, A6, A7, A8, A9, AA, AB, AC, AD, AE, AF, AG, AH, AI, AJ, AK, AL, AM, AN, AO, AP, AQ, AR, AS, AT, AU, AV, AW, AX, AY
Molecule details ›
Chains: A1, A2, A3, A4, A5, A6, A7, A8, A9, AA, AB, AC, AD, AE, AF, AG, AH, AI, AJ, AK, AL, AM, AN, AO, AP, AQ, AR, AS, AT, AU, AV, AW, AX, AY
Length: 560 amino acids
Theoretical weight: 61.3 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
UniProt:
  • Canonical: P15928 (Residues: 1-560; Coverage: 100%)
Gene names: STM1969, fla AII.1, fla BI, fliF
Sequence domains:
Flagellar motor switch protein FliG Chains: B1, B2, B3, B4, B5, B6, B7, B8, B9, BA, BB, BC, BD, BE, BF, BG, BH, BI, BJ, BK, BL, BM, BN, BO, BP, BQ, BR, BS, BT, BU, BV, BW, BX, BY
Molecule details ›
Chains: B1, B2, B3, B4, B5, B6, B7, B8, B9, BA, BB, BC, BD, BE, BF, BG, BH, BI, BJ, BK, BL, BM, BN, BO, BP, BQ, BR, BS, BT, BU, BV, BW, BX, BY
Length: 331 amino acids
Theoretical weight: 36.89 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
UniProt:
  • Canonical: P0A1J9 (Residues: 1-331; Coverage: 100%)
Gene names: STM1970, fla AII.2, fla BII, fliG
Sequence domains:
Flagellar motor switch protein FliM Chains: C1, C2, C3, C4, C5, C6, C7, C8, C9, CA, CB, CC, CD, CE, CF, CG, CH, CI, CJ, CK, CL, CM, CN, CO, CP, CQ, CR, CS, CT, CU, CV, CW, CX, CY
Flagellar motor switch protein FliN Chains: D1, D2, D3, D4, D5, D6, D7, D8, D9, DA, DB, DC, DD, DE, DF, DG, DH, DI, DJ, DK, DL, DM, DN, DO, DP, DQ, DR, DS, DT, DU, DV, DW, DX, DY, E1, E2, E3, E4, E5, E6, E7, E8, E9, EA, EB, EC, ED, EE, EF, EG, EH, EI, EJ, EK, EL, EM, EN, EO, EP, EQ, ER, ES, ET, EU, EV, EW, EX, EY, F1, F2, F3, F4, F5, F6, F7, F8, F9, FA, FB, FC, FD, FE, FF, FG, FH, FI, FJ, FK, FL, FM, FN, FO, FP, FQ, FR, FS, FT, FU, FV, FW, FX, FY

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.6Å
Relevant EMDB volumes: EMD-42439

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