› 8uoz

Solution NMR

EmrE structure in the TPP-bound state (WT/E14Q heterodimer)

Released:
DOI: 10.2210/pdb8uoz/pdb
PDB entry 8uoz coloured by chain, ensemble of 10 models, front view.
PDB entry 8uoz coloured by chain, ensemble of 10 models, side view.
PDB entry 8uoz coloured by chain, ensemble of 10 models, top view.
Source organism: Escherichia coli
Primary publication:
Dynamics underlie the drug recognition mechanism by the efflux transporter EmrE.
Li J, Her AS, Besch A, Ramirez-Cordero B, Crames M, Banigan JR, Mueller C, Marsiglia WM, Zhang Y, Traaseth NJ
OpenAccess logo Nat Commun 15 4537 (2024)
PMID: 38806470

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-273406 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Multidrug transporter EmrE Chain: A
Molecule details ›
Chain: A
Length: 110 amino acids
Theoretical weight: 11.96 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P23895 (Residues: 1-110; Coverage: 100%)
Gene names: JW0531, b0543, eb, emrE, mvrC
Sequence domains: Small Multidrug Resistance protein
Multidrug transporter EmrE Chain: B
Molecule details ›
Chain: B
Length: 110 amino acids
Theoretical weight: 11.96 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P23895 (Residues: 1-110; Coverage: 100%)
Gene names: JW0531, b0543, eb, emrE, mvrC
Sequence domains: Small Multidrug Resistance protein

Ligands and Environments

1 bound ligand:
Ligand P4P 1 x P4P
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 32%
Refinement method: simulated annealing
Expression system: Escherichia coli

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