PDB 8upo structure summary ‹ Protein Data Bank in Europe (PDBe)

Function and Biology Details

Reaction catalysed:

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

Biochemical function:

structural constituent of ribosome

Biological process:

translation

Cellular component:

ribonucleoprotein complex

Sequence domains:

Structure analysis Details

Assembly composition:

hetero 66-mer (preferred)

PDBe Complex ID:

PDB-CPX-244895 (preferred)

Entry contents:

57 distinct polypeptide molecules
2 distinct DNA molecules
5 distinct RNA molecules

Macromolecules (64 distinct):

Ribosomal protein L21 Chain: 0

Molecule details ›

Chain: 0
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Large ribosomal subunit protein uL22 Chain: 1

Molecule details ›

Chain: 1
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P61175 (Residues: 1-110; Coverage: 100%)

Gene names: JW3277, b3315, eryB, rplV
Sequence domains: Ribosomal protein L22p/L17e

50S ribosomal protein L23 Chain: 2

Molecule details ›

Chain: 2
Length: 100 amino acids
Theoretical weight: 11.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

50S ribosomal protein L24 Chain: 3

Molecule details ›

Chain: 3
Length: 104 amino acids
Theoretical weight: 11.34 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

50S ribosomal protein L25 Chain: 4

Molecule details ›

Chain: 4
Length: 94 amino acids
Theoretical weight: 10.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Large ribosomal subunit protein uL10 Chain: 9

Molecule details ›

Chain: 9
Length: 165 amino acids
Theoretical weight: 17.74 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P0A7J3 (Residues: 1-165; Coverage: 100%)

Gene names: JW3948, b3985, rplJ
Sequence domains: Ribosomal protein L10

DNA-directed RNA polymerase subunit beta Chain: AA

Molecule details ›

Chain: AA
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)

Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:

Transcription antitermination protein RfaH Chain: AB

Molecule details ›

Chain: AB
Length: 162 amino acids
Theoretical weight: 18.36 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: C3SK02 (Residues: 1-162; Coverage: 100%)

Gene names: ABE91_020680, ABT96_004855, ACN81_23950, ACU57_13495, ACW72_004680, ATM34_003236, AW118_25355, B6R15_004015, BANRA_02505, BANRA_04904, BER14_25710, BG944_003673, BGM66_003203, BGZ_01977, BGZ_04739, BJI68_05490, BK300_22455, BK383_22470, BMT50_09200, BRV02_002711, BTB68_001947, BTQ06_27030, BVE17_004577, BXT93_06440, BvCmsKKP061_01522, BvCmsNSP007_02672, C0P57_000544, C1Q91_004262, C2M16_20855, C3F40_16460, C4M78_23240, C719_004332, C7B02_20725, C9114_17820, C9160_17475, C9Z68_23120, CF22_003752, CG704_18665, CIG67_07875, CLG78_004625, CQ842_11205, CQ842_16970, CR538_25020, CTR35_002422, CV628_003140, CV83915_02225, CWS33_20840, D4M65_18945, DAH27_20510, DIV22_24040, DL738_18550, DL968_14380, DNQ45_14650, DNX30_19790, DS732_00760, DTL43_17155, DU321_17035, E0I52_22015, E2865_04815, E4K51_16320, E5H86_14925, E6D34_09585, EAI46_16740, ECs4770, EIA08_15810, EN85_003116, EPS97_18175, F7G01_20535, F9413_11110, F9461_20550, FEH53_16265, FGAF1022_45910, FGAF848_46040, FIJ20_20025, FJQ40_20750, FKO60_20130, FOI11_016430, FOI11_06750, FORC44_5467, FPS11_16060, FVB16_11915, FWK02_07980, FZU14_23195, G3V95_18695, G4A38_20845, G4A47_20625, G5603_18205, G6Z99_17770, GAI89_19520, GAJ12_16765, GKF66_21335, GNW61_07645, GNZ05_20625, GOP25_17800, GP965_13065, GP979_14335, GQE86_18165, GQM04_09440, GQM13_22115, GQM21_14015, GQN24_17135, GRW05_24980, GRW24_08980, GTP92_17360, GUC01_20805, H0O72_20610, HEP30_018290, HEP34_003121, HHH44_003630, HIA71_17845, HID72_003656, HJ488_003073, HJS37_004001, HKA49_003720, HMV95_12890, HND12_25375, HV109_23350, HVW04_14630, HVW43_15765, HVW90_13410, HVY77_25085, I6H00_17950, I6H02_14765, IFN33_002872, IH772_01905, J0541_003579, J5U05_003801, J8F57_003373, JNP96_27275, KQO22_003555, KTF03_002451, NCTC10082_02242, NCTC10429_00323, NCTC10865_06093, NCTC10974_05576, NCTC11181_02169, NCTC11341_02591, NCTC13148_04204, NCTC8009_08655, NCTC8179_05280, NCTC8500_05515, NCTC8960_02506, NCTC8985_04237, NCTC9045_05746, NCTC9073_01050, NCTC9117_06165, NCTC9703_04383, NCTC9706_02164, NCTC9962_03384, OFN31_18435, P6223_002431, QDW62_25230, RZR61_20645, SAMEA3472044_03799, SAMEA3472112_03195, SAMEA3752557_03881, TUM18780_43110, WR15_15095, rfaH, rfaH_1, rfaH_2
Sequence domains: Transcription termination factor nusG

DNA-directed RNA polymerase subunit alpha Chains: AC, AD

Molecule details ›

Chains: AC, AD
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P0A7Z4 (Residues: 1-329; Coverage: 100%)

Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:

DNA-directed RNA polymerase subunit beta' Chain: AE

Molecule details ›

Chain: AE
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P0A8T7 (Residues: 1-1407; Coverage: 100%)

Gene names: JW3951, b3988, rpoC, tabB
Sequence domains:

Small ribosomal subunit protein bS18 Chain: C

Molecule details ›

Chain: C
Length: 75 amino acids
Theoretical weight: 9.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: A0A0E2KXL3 (Residues: 1-75; Coverage: 100%)

Gene names: G925_04532, rpsR
Sequence domains: Ribosomal protein S18

Small ribosomal subunit protein bS20 Chain: E

Molecule details ›

Chain: E
Length: 87 amino acids
Theoretical weight: 9.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: C3TRH7 (Residues: 1-87; Coverage: 100%)

Gene names: A2J79_001479, A5U30_000450, ABE91_002840, ABT96_000180, ACN81_29300, ACU57_08035, ACW72_001888, ATM34_003221, AW118_10515, AWP47_04800, B6R15_000372, B6R31_002612, BANRA_00498, BANRA_03198, BCB93_001630, BE932_23130, BER14_08565, BG944_002014, BGM66_001982, BGZ_00149, BGZ_01355, BJI68_23170, BK300_10160, BK383_13810, BKL28_001230, BMT50_04750, BRV02_001727, BTB68_000387, BTQ06_20830, BVE17_000902, BXT93_08445, BvCmsKKP061_01774, BvCmsNSP007_03910, C0P57_001724, C1Q91_004755, C2M16_10025, C2R31_000773, C3F40_13025, C4M78_04050, C719_003247, C7B02_09940, C9114_07750, C9160_20885, C9Z68_04045, CF22_002905, CG704_02485, CIG67_13240, CLG78_001275, CQ842_03615, CQ842_07950, CR538_21410, CTR35_001700, CV628_004200, CWS33_08845, D3C88_27595, D3G36_11455, D4M65_00930, D4N09_07610, D9D43_03045, D9E49_02920, D9J61_10145, DAH27_12615, DD762_00305, DIV22_05480, DL738_02510, DL968_13525, DNQ45_08590, DNX30_03070, DS732_05000, DTL43_06695, DU321_02915, E0I52_18085, E2865_00020, E4K51_06530, E5H86_10235, E6D34_22220, EAI46_14690, ECs0026, EIA08_05570, EIMP300_82500, EIZ93_24750, EN85_004084, EPS76_15825, EPS97_00330, EWK56_14125, ExPECSC038_04138, F7F11_02830, F7G01_01915, F7N46_19400, F9413_03990, F9461_05560, F9B07_04255, FEH53_10475, FGAF1022_39860, FGAF848_49570, FIJ20_22060, FJQ40_06740, FKO60_25310, FOI11_013580, FOI11_22100, FPI65_00060, FPS11_05665, FTV93_14405, FVB16_23965, FWK02_19890, FZU14_06515, G3V95_03165, G3W53_01275, G4A38_11000, G4A47_11230, G5603_15185, G6Z99_21090, GAI89_11025, GAJ12_06670, GGB84_001424, GKF66_03250, GNW61_12150, GNZ05_10535, GOP25_07970, GP711_08775, GP965_17595, GP975_22055, GQE86_11535, GQM04_03640, GQM13_11900, GQM21_07545, GQN24_20555, GQN34_16940, GRW05_18565, GRW24_19260, GTP92_17315, GUC01_17160, H0O72_17735, HEP30_002010, HEP34_002732, HHH44_001269, HI055_000925, HI084_000004, HIA71_06060, HID72_001632, HIE29_002940, HJ488_000651, HJQ60_000838, HJS37_001773, HKA49_001794, HLX92_13830, HLZ39_05560, HLZ50_14320, HMV95_05330, HND12_09605, HV109_20105, HV209_00820, HV209_22380, HVV39_16845, HVW04_10975, HVW43_12510, HVW90_10320, HVY77_21905, I6H00_14880, I6H02_17860, IFC14_000185, IFN33_000180, IG021_000794, IH772_13695, IT029_004745, J0541_002476, J5U05_003239, J8F57_002267, JNP96_04295, KQO22_001152, KTF03_000515, NCTC10082_01545, NCTC10089_04234, NCTC10418_06301, NCTC10429_04410, NCTC10764_05953, NCTC10767_03791, NCTC10865_05258, NCTC10974_04789, NCTC11112_03207, NCTC11126_03412, NCTC11181_01521, NCTC11341_03418, NCTC13148_04985, NCTC7922_05631, NCTC7927_04659, NCTC8009_07557, NCTC8179_01182, NCTC8500_04735, NCTC8621_04353, NCTC8622_02456, NCTC8959_04686, NCTC8960_01711, NCTC8985_03329, NCTC9044_02991, NCTC9045_04893, NCTC9075_05681, NCTC9077_05304, NCTC9702_04945, NCTC9703_03586, NCTC9706_01568, NCTC9962_05928, OFN31_13695, OGM49_03330, P6223_000568, PWL68_003118, QDW62_22405, R8G00_25790, R8O40_001206, RZR61_05785, SAMEA3472044_01892, SAMEA3472112_00142, TUM18780_37490, WR15_01590, rpsT
Sequence domains: Ribosomal protein S20

Small ribosomal subunit protein bS21 Chain: F

Molecule details ›

Chain: F
Length: 71 amino acids
Theoretical weight: 8.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: A0A0E2L2J1 (Residues: 1-71; Coverage: 100%)

Gene names: G925_03304, rpsU
Sequence domains: Ribosomal protein S21

Small ribosomal subunit protein uS2 Chain: G

Molecule details ›

Chain: G
Length: 241 amino acids
Theoretical weight: 26.78 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: C3TPN2 (Residues: 1-241; Coverage: 100%)

Gene names: A2J79_001332, A5U30_000304, ABT96_000033, ACW72_001741, AW118_17855, AWP47_05585, B6R15_000228, B6R31_002468, BANRA_03339, BCB93_004076, BE932_23980, BER14_07785, BGM66_002005, BGZ_00295, BGZ_01212, BJI68_15640, BK383_20240, BKL28_001082, BTB68_000239, BXT93_00705, BvCmsNSP007_03764, C0P57_001478, C2M16_10795, C2R31_000627, C3F40_12245, C4M78_19960, C719_003101, C9Z68_04785, CG704_12155, CIG67_16575, CLG78_001128, CTR35_003018, CV628_000439, D3G36_16675, D4M65_00165, D4N09_06800, D9D43_02305, D9E49_02145, D9J61_11510, DD762_01090, DL738_01740, DL968_03335, DNX30_02270, DTL43_05925, DU321_03650, E4K51_05790, E6D34_00990, ECs0171, EF082_10775, EIA08_06325, EIZ93_07925, EPS76_09885, EPS97_01065, EWK56_14885, ExPECSC038_03990, F7F11_02075, F7G01_02645, F7N46_12615, F9413_16860, F9461_04820, F9B07_03520, FEH53_10900, FGAF1022_38460, FGAF848_51030, FIJ20_13895, FKO60_12340, FOI11_012840, FOI11_22840, FPI65_00925, FPS11_04930, FZU14_18260, G3V95_02460, G3W53_17935, G4A38_19340, G4A47_18775, G5603_21760, G6Z99_21820, GGB84_001276, GKF66_15185, GNW61_11380, GOP25_07250, GP711_09530, GP975_11310, GQA06_13075, GQE86_00825, GQM13_12620, GQM21_02780, GQN34_16185, GRW05_07695, GTP92_20145, H0O72_10185, HEP30_016685, HEP34_000949, HI055_000776, HI084_000157, HIE29_003103, HJQ60_000692, HJS37_001627, HLX92_18970, HLZ39_04825, HLZ50_06960, HMV95_04585, HND12_10355, HV109_19380, HV209_01540, HVV39_17630, HVW04_10225, HVW43_11755, HVW90_09585, I6H00_14085, IFC14_000033, IFN33_000033, IG021_000642, IH772_14435, IT029_001942, J0541_003915, JNP96_05035, KTF03_000366, NCTC10082_01383, NCTC10089_04076, NCTC10767_03962, NCTC10974_04616, NCTC11126_03634, NCTC13148_05196, NCTC7922_05813, NCTC7927_04507, NCTC7928_04613, NCTC8500_04546, NCTC8621_04202, NCTC8959_04843, NCTC9044_03184, NCTC9045_04711, NCTC9117_05053, NCTC9702_04760, NCTC9706_01337, OGM49_04160, P6223_002958, PWL68_003270, QDW62_21670, R8G00_25015, R8O40_001042, RZR61_12600, SAMEA3472044_04157, SAMEA3472112_04653, SAMEA3752557_00874, TUM18780_36030, rpsB
Sequence domains: Ribosomal protein S2

30S ribosomal protein S1 Chain: H

Molecule details ›

Chain: H
Length: 557 amino acids
Theoretical weight: 61.24 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Small ribosomal subunit protein uS3 Chain: I

Molecule details ›

Chain: I
Length: 233 amino acids
Theoretical weight: 26.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: B7MCS9 (Residues: 1-233; Coverage: 100%)

Gene names: ECS88_3701, rpsC
Sequence domains:

Small ribosomal subunit protein uS4 Chain: J

Molecule details ›

Chain: J
Length: 206 amino acids
Theoretical weight: 23.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P0A7V8 (Residues: 1-206; Coverage: 100%)

Gene names: JW3258, b3296, ramA, rpsD
Sequence domains:

Small ribosomal subunit protein uS5 Chain: K

Molecule details ›

Chain: K
Length: 167 amino acids
Theoretical weight: 17.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P0A7W1 (Residues: 1-167; Coverage: 100%)

Gene names: JW3265, b3303, rpsE, spc
Sequence domains:

Small ribosomal subunit protein bS6 Chain: L

Molecule details ›

Chain: L
Length: 135 amino acids
Theoretical weight: 15.73 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P02358 (Residues: 1-135; Coverage: 100%)

Gene names: JW4158, b4200, rpsF
Sequence domains: Ribosomal protein S6

Small ribosomal subunit protein uS7 Chain: M

Molecule details ›

Chain: M
Length: 179 amino acids
Theoretical weight: 20.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P02359 (Residues: 1-179; Coverage: 100%)

Gene names: JW3303, b3341, rpsG
Sequence domains: Ribosomal protein S7p/S5e

30S ribosomal protein S8 Chain: N

Molecule details ›

Chain: N
Length: 130 amino acids
Theoretical weight: 14.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

30S ribosomal protein S9 Chain: O

Molecule details ›

Chain: O
Length: 130 amino acids
Theoretical weight: 14.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

30S ribosomal protein S10 Chain: P

Molecule details ›

Chain: P
Length: 103 amino acids
Theoretical weight: 11.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Small ribosomal subunit protein uS11 Chain: Q

Molecule details ›

Chain: Q
Length: 129 amino acids
Theoretical weight: 13.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: B7MCR3 (Residues: 1-129; Coverage: 100%)

Gene names: ECS88_3685, rpsK
Sequence domains: Ribosomal protein S11

Small ribosomal subunit protein uS12 Chain: R

Molecule details ›

Chain: R
Length: 124 amino acids
Theoretical weight: 13.77 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P0A7S3 (Residues: 1-124; Coverage: 100%)

Gene names: JW3304, b3342, rpsL, strA
Sequence domains: Ribosomal protein S12/S23

30S ribosomal protein S14 Chain: S

Molecule details ›

Chain: S
Length: 101 amino acids
Theoretical weight: 11.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

30S ribosomal protein S15 Chain: T

Molecule details ›

Chain: T
Length: 89 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Small ribosomal subunit protein bS16 Chain: U

Molecule details ›

Chain: U
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: C3SYP2 (Residues: 1-82; Coverage: 100%)

Gene names: A2J79_004025, A5U30_004754, ABE91_013510, ABT96_004672, ACN81_10155, ACU57_00080, ACW72_004544, ATM34_004532, AW118_18435, AWP47_02660, B6R15_004151, B6R31_004752, BANRA_01153, BANRA_03473, BCB93_004648, BE932_10860, BER14_21265, BG944_004814, BGM66_003574, BGZ_03315, BGZ_03367, BJI68_05000, BK300_25595, BK383_10930, BKL28_002032, BMT50_17425, BRV02_004538, BTB68_003665, BTQ06_08500, BVE17_004306, BXT93_09655, BvCmsKKP061_02398, BvCmsNSP007_04560, C0P57_003571, C1Q91_004654, C2M16_24775, C2R31_001293, C3F40_23855, C4M78_00095, C719_003857, C7B02_26315, C9114_19790, C9160_23660, C9Z68_21275, CF22_004793, CG704_21555, CIG67_05880, CLG78_004553, CQ842_19340, CQ842_21890, CR538_06305, CTR35_004184, CV628_003833, CV83915_03504, CWS33_25635, D3C88_03060, D3G36_24900, D4M65_08425, D4N09_19000, D9D43_23095, D9E49_21005, D9J61_07050, DAH27_25645, DD762_23905, DIV22_27270, DL738_24805, DL968_07140, DNQ45_01400, DNX30_24590, DS732_19205, DTL43_21645, DU321_24440, E0I52_20340, E2865_03661, E4K51_10645, E5H86_23225, E6D34_21955, EAI46_08750, EAI46_19150, ECs3472, EF082_15670, EIA08_06550, EIMP300_31950, EIZ93_15670, EN85_004117, EPS76_07525, EPS97_18825, EWK56_06055, ExPECSC038_01348, F7F11_01840, F7G01_23475, F7N46_19970, F9413_21435, F9461_09670, F9B07_09610, FEH53_11955, FGAF1022_12690, FGAF848_35260, FIJ20_21775, FJQ40_23010, FKO60_26240, FOI11_00085, FOI11_023095, FPI65_16330, FPS11_21845, FVB16_19505, FWK02_06545, FZU14_20420, G3V95_09900, G4A38_21035, G4A47_01280, G5603_07605, G6Z99_06215, GAI89_21540, GAJ12_00085, GGB84_004394, GKF66_23925, GNW61_16330, GNZ05_25610, GOP25_24505, GP711_05970, GP965_09730, GP975_09855, GP979_11870, GQE86_17960, GQM04_19120, GQM13_06275, GQM21_13480, GQN24_22215, GQN34_14095, GRW05_07330, GRW24_07355, GTP92_23395, GUC01_18090, H0O72_19850, HEP30_021265, HEP34_004233, HHH44_004385, HI055_003780, HI084_003760, HIA71_04170, HID72_004041, HIE29_001459, HJ488_002399, HJQ60_000018, HJS37_004785, HKA49_004932, HLX92_22510, HLZ39_14795, HLZ50_20265, HMV95_03235, HND12_25480, HV109_05690, HV209_12690, HVV39_05045, HVW04_21985, HVW43_23290, HVW90_19965, HVY77_06760, I6H00_24675, I6H02_08660, IFC14_000812, IFN33_004700, IG021_002875, IH772_08265, IT029_001737, J0541_004264, J5U05_003371, J8F57_004160, JNP96_20830, KQO22_003452, KTF03_001096, NCTC10082_03675, NCTC10089_01224, NCTC10418_01999, NCTC10429_01986, NCTC10764_00466, NCTC10767_02511, NCTC10865_01552, NCTC10974_01459, NCTC11112_07458, NCTC11126_05241, NCTC11181_03429, NCTC11341_01001, NCTC13148_02463, NCTC7922_00783, NCTC7927_01359, NCTC8009_02720, NCTC8179_06855, NCTC8500_01313, NCTC8959_02554, NCTC8960_03978, NCTC8985_06048, NCTC9044_00378, NCTC9045_01451, NCTC9073_04663, NCTC9075_01815, NCTC9077_01607, NCTC9081_05689, NCTC9702_01486, NCTC9703_05790, NCTC9706_03497, NCTC9962_00822, OFN31_20550, OGM49_18375, P6223_004685, PWL68_001675, QDW62_06175, R8G00_07990, R8O40_004589, RZR61_19260, SAMEA3472044_03967, SAMEA3472112_00948, SAMEA3752557_04552, TUM18780_10930, WR15_13330, rpsP
Sequence domains: Ribosomal protein S16

30S ribosomal protein S17 Chain: V

Molecule details ›

Chain: V
Length: 84 amino acids
Theoretical weight: 9.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

30S ribosomal protein S19 Chain: W

Molecule details ›

Chain: W
Length: 92 amino acids
Theoretical weight: 10.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Small ribosomal subunit protein uS13 Chain: X

Molecule details ›

Chain: X
Length: 118 amino acids
Theoretical weight: 13.13 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: A0A7U9IV78 (Residues: 1-118; Coverage: 100%)

Gene names: G711_03878, rpsM
Sequence domains: Ribosomal protein S13/S18

Large ribosomal subunit protein uL11 Chain: Y

Molecule details ›

Chain: Y
Length: 142 amino acids
Theoretical weight: 14.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P0A7J7 (Residues: 1-142; Coverage: 100%)

Gene names: JW3946, b3983, relC, rplK
Sequence domains:

50S ribosomal protein L7/L12 Chain: Z

Molecule details ›

Chain: Z
Length: 121 amino acids
Theoretical weight: 12.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Large ribosomal subunit protein bL27 Chain: b

Molecule details ›

Chain: b
Length: 85 amino acids
Theoretical weight: 9.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P0A7L8 (Residues: 1-85; Coverage: 100%)

Gene names: JW3152, b3185, rpmA
Sequence domains: Ribosomal L27 protein

Large ribosomal subunit protein bL28 Chain: c

Molecule details ›

Chain: c
Length: 78 amino acids
Theoretical weight: 9.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: A0A1X3JA73 (Residues: 1-78; Coverage: 100%)

Gene names: ECVG_03476, rpmB
Sequence domains: Ribosomal L28 family

50S ribosomal protein L29 Chain: e

Molecule details ›

Chain: e
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

50S ribosomal protein L30 Chain: f

Molecule details ›

Chain: f
Length: 59 amino acids
Theoretical weight: 6.55 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Large ribosomal subunit protein bL31 Chain: g

Molecule details ›

Chain: g
Length: 70 amino acids
Theoretical weight: 7.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P0A7M9 (Residues: 1-70; Coverage: 100%)

Gene names: JW3907, b3936, rpmE
Sequence domains: Ribosomal protein L31

Large ribosomal subunit protein uL2 Chain: h

Molecule details ›

Chain: h
Length: 273 amino acids
Theoretical weight: 29.92 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P60422 (Residues: 1-273; Coverage: 100%)

Gene names: JW3279, b3317, rplB
Sequence domains:

50S ribosomal protein L32 Chain: i

Molecule details ›

Chain: i
Length: 57 amino acids
Theoretical weight: 6.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Large ribosomal subunit protein uL3 Chain: j

Molecule details ›

Chain: j
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P60438 (Residues: 1-209; Coverage: 100%)

Gene names: JW3282, b3320, rplC
Sequence domains: Ribosomal protein L3

50S ribosomal protein L33 Chain: k

Molecule details ›

Chain: k
Length: 55 amino acids
Theoretical weight: 6.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Large ribosomal subunit protein uL4 Chain: l

Molecule details ›

Chain: l
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: A1AGK6 (Residues: 1-201; Coverage: 100%)

Gene names: APECO1_3132, Ecok1_33020, rplD
Sequence domains: Ribosomal protein L4/L1 family

Large ribosomal subunit protein bL34 Chain: m

Molecule details ›

Chain: m
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: B7MGC4 (Residues: 1-46; Coverage: 100%)

Gene names: ECS88_4126, rpmH
Sequence domains: Ribosomal protein L34

50S ribosomal protein L5 Chain: n

Molecule details ›

Chain: n
Length: 179 amino acids
Theoretical weight: 20.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Large ribosomal subunit protein bL35 Chain: o

Molecule details ›

Chain: o
Length: 65 amino acids
Theoretical weight: 7.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P0A7Q1 (Residues: 1-65; Coverage: 100%)

Gene names: JW1707, b1717, rpmI
Sequence domains: Ribosomal protein L35

Large ribosomal subunit protein uL6 Chain: p

Molecule details ›

Chain: p
Length: 177 amino acids
Theoretical weight: 18.93 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: C3SR17 (Residues: 1-177; Coverage: 100%)

Gene names: A2J79_004074, A5U30_004539, ABE91_009085, ABT96_004807, ACN81_25455, ACU57_21025, ACW72_000027, ATM34_000472, AW118_24835, AWP47_12710, B6R15_004082, B6R31_004981, BANRA_01936, BANRA_04315, BCB93_004280, BE932_15470, BER14_25125, BG944_001120, BGM66_003606, BGZ_02603, BGZ_04112, BJI68_08740, BK300_26865, BK383_24710, BKL28_004556, BMT50_12280, BRV02_004610, BTB68_004369, BTQ06_10625, BVE17_004507, BXT93_05540, BvCmsKKP061_03017, BvCmsNSP007_03998, C0P57_002655, C1Q91_004917, C2M16_25470, C2R31_004620, C3F40_19785, C4M78_26370, C719_004900, C7B02_26515, C9114_23825, C9160_24335, C9Z68_22570, CF22_004862, CG704_20935, CIG67_10740, CLG78_004510, CQ842_14390, CQ842_22280, CR538_02485, CTR35_003617, CV628_002837, CWS33_24875, D3G36_23115, D4M65_20590, D4N09_20190, D9D43_22615, D9E49_05175, D9J61_16670, DAH27_08440, DD762_23565, DIV22_15240, DL738_24100, DL968_17150, DNQ45_03775, DNX30_25460, DS732_24280, DTL43_20980, DU321_12020, E0I52_20160, E2865_04458, E4K51_21510, E5H86_24505, E6D34_21740, EAI46_07495, ECs4170, EF082_25230, EIZ93_12450, EN85_004362, EPS76_07195, EPS97_20075, EWK56_25015, ExPECSC038_03653, F7F11_22705, F7G01_23675, F7N46_23990, F9413_21215, F9461_25635, F9B07_24665, FGAF1022_04760, FGAF848_25980, FIJ20_21175, FJQ40_18325, FKO60_25655, FOI11_019240, FOI11_03940, FPI65_20325, FPS11_25410, FTV93_20880, FVB16_04890, FWK02_06290, FZU14_21840, G3V95_19865, G3W53_20975, G4A38_21530, G4A47_20800, G6Z99_02060, GAI89_24810, GAJ12_24700, GKF66_21565, GNW61_16650, GNZ05_25965, GOP25_22725, GP711_23325, GP954_00925, GP965_04315, GP975_01205, GP979_01985, GQA06_03930, GQE86_20100, GQM04_10585, GQM13_25220, GQM21_11250, GQN24_22695, GQN34_23285, GRC73_21730, GRW05_09080, GRW24_04735, GTP92_23175, GUC01_21200, H0O72_19435, HEP30_018655, HEP34_004767, HHH44_004532, HI055_004143, HI084_004377, HIA71_24770, HID72_004557, HIE29_005190, HJ488_004883, HJQ60_005008, HJS37_004704, HKA49_005032, HLX92_10055, HLZ39_22195, HLZ50_22160, HMV95_19625, HV109_02170, HV209_14695, HVV39_09285, HVW43_18650, HVW90_16435, HVY77_02155, I6H00_20905, I6H02_12010, IFC14_004403, IFN33_004912, IG021_003341, IH772_23995, IT029_004950, J0541_004400, J5U05_004084, J8F57_004250, JNP96_25325, KQO22_004050, KTF03_004682, NCTC10082_02836, NCTC10089_00499, NCTC10418_00687, NCTC10764_03860, NCTC10767_01513, NCTC11112_01039, NCTC11126_02768, NCTC11181_02735, NCTC11341_01892, NCTC13148_03444, NCTC7927_00548, NCTC7928_02519, NCTC8009_01508, NCTC8621_00505, NCTC8959_03264, NCTC8960_03067, NCTC8985_04979, NCTC9001_04251, NCTC9044_01360, NCTC9077_00601, NCTC9081_00914, NCTC9117_00753, NCTC9702_00534, NCTC9703_04963, NCTC9706_02734, OFN31_22800, OGM49_22350, P6223_003986, PWL68_004852, PWL68_005333, QDW62_02175, R8G00_13260, R8O40_004924, RZR61_12910, SAMEA3472044_01159, SAMEA3472112_00690, SAMEA3752557_01955, TUM18780_04330, WR15_19625, rplF
Sequence domains: Ribosomal protein L6

Large ribosomal subunit protein bL36 Chain: q

Molecule details ›

Chain: q
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: A0A0E2L017 (Residues: 1-38; Coverage: 100%)

Gene names: G925_03522, rpmJ
Sequence domains: Ribosomal protein L36

50S ribosomal protein L9 Chain: r

Molecule details ›

Chain: r
Length: 149 amino acids
Theoretical weight: 15.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

50S ribosomal protein L13 Chain: s

Molecule details ›

Chain: s
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Large ribosomal subunit protein uL14 Chain: t

Molecule details ›

Chain: t
Length: 123 amino acids
Theoretical weight: 13.57 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: S1NW45 (Residues: 1-123; Coverage: 100%)

Gene names: A13A_03633, rplN
Sequence domains: Ribosomal protein L14p/L23e

Large ribosomal subunit protein uL15 Chain: u

Molecule details ›

Chain: u
Length: 144 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: A0A037Y8L6 (Residues: 1-144; Coverage: 100%)

Gene names: ACU57_21045, BANRA_01932, BGM66_003610, BJI68_08760, C0P57_002651, CIG67_10760, CTR35_003613, DL968_17170, DTL43_21000, E4K51_21490, EIZ93_12470, FOI11_019260, FOI11_03920, FWK02_06270, G3V95_19845, G4A38_21510, G4A47_20780, GNW61_16630, GOP25_22705, GP954_00945, GP965_04295, GP979_02005, GRW05_09060, HMV95_19605, HVY77_02175, IH772_24015, J0541_004396, JNP96_25305, NCTC11126_02772, NCTC9045_00567, NCTC9117_00757, NCTC9706_02738, P6223_003982, QDW62_02195, RZR61_12890, rplO
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A

Large ribosomal subunit protein uL16 Chain: v

Molecule details ›

Chain: v
Length: 136 amino acids
Theoretical weight: 15.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: P0ADY7 (Residues: 1-136; Coverage: 100%)

Gene names: JW3275, b3313, rplP
Sequence domains: Ribosomal protein L16p/L10e

50S ribosomal protein L17 Chain: w

Molecule details ›

Chain: w
Length: 127 amino acids
Theoretical weight: 14.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

50S ribosomal protein L18 Chain: x

Molecule details ›

Chain: x
Length: 117 amino acids
Theoretical weight: 12.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

50S ribosomal protein L19 Chain: y

Molecule details ›

Chain: y
Length: 115 amino acids
Theoretical weight: 13.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Large ribosomal subunit protein bL20 Chain: z

Molecule details ›

Chain: z
Length: 118 amino acids
Theoretical weight: 13.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:

Canonical: B7MAS6 (Residues: 1-118; Coverage: 100%)

Gene names: ECS88_1766, rplT
Sequence domains: Ribosomal protein L20

NT DNA with ops signal Chain: 5

Molecule details ›

Chain: 5
Length: 38 nucleotides
Theoretical weight: 11.79 KDa

T DNA Chain: 6

Molecule details ›

Chain: 6
Length: 38 nucleotides
Theoretical weight: 11.53 KDa

mRNA with 21 nt long spacer Chain: 7

Molecule details ›

Chain: 7
Length: 38 nucleotides
Theoretical weight: 11.99 KDa

E-site and P-site tRNA (fMet) Chains: A, B

Molecule details ›

Chains: A, B
Length: 76 nucleotides
Theoretical weight: 24.5 KDa
Sequence domains: tRNA

16S ribosomal RNA Chain: D

Molecule details ›

Chain: D
Length: 1542 nucleotides
Theoretical weight: 499.69 KDa
Sequence domains: Bacterial small subunit ribosomal RNA

23S ribosomal RNA Chain: a

Molecule details ›

Chain: a
Length: 2904 nucleotides
Theoretical weight: 941.64 KDa
Sequence domains: Bacterial large subunit ribosomal RNA

5S ribosomal RNA Chain: d

Molecule details ›

Chain: d
Length: 120 nucleotides
Theoretical weight: 38.79 KDa
Sequence domains: 5S ribosomal RNA

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Resolution: 5.5Å

Relevant EMDB volumes: EMD-42453

Expression system: Escherichia coli

Protein Data Bank in Europe

Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome

Function and Biology Details

Structure analysis Details

Ligands and Environments

Experiments and Validation Details

Quick links

PDBe › 8upo

Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome

Function and Biology Details

Structure analysis Details

Ligands and Environments

Experiments and Validation Details

Quick links